【作者】 王飞；

【导师】 李引乾；

【作者基本信息】 西北农林科技大学 ， 兽医硕士（专业学位）， 2019， 硕士

【摘要】 目的:七清败毒散的主要成分有:生石膏,金银花,鱼腥草,板蓝根,忍冬藤,贯众,甘草等。本研究对七清败毒散进行了药效学实验和急性毒性试验,为今后七清败毒散对猪繁殖与呼吸综合征的临床应用提供理论依据。方法:药效学试验:采用蛋白胨制成发热模型研究七清败毒散的解热作用;采用二甲苯致小鼠耳廓炎症水肿法研究其抗炎作用;选用金黄色葡萄球菌、大肠埃希菌、沙门氏菌作为实验用菌,采用肉汤试管稀释法测定七清败毒散对这3种常见菌最小抑菌浓度(MIC)。在96孔细胞培养板上上测定七清败毒散在细胞水平上对PRRSV抑制作用。结果:七清败毒散能够显著抑制蛋白胨所致家兔体温升高,能明显减轻二甲苯所导致的小鼠耳肿胀,对常见的病原菌大肠埃希菌、沙门氏菌、金黄色葡萄球菌均有一定的抑制作用,在细胞上对PRRSV有明显的抑制作用。结论:七清败毒散的解热、抗炎、抑菌和抗病毒作用良好。更多还原

【Abstract】 Objective: Qiqingbaidu powder adopts three classic drug pair of traditional Chinese medicine: raw gypsum-honeysuckle rattan,honeysuckle-houttuynia houttuyniae and honeysuckle-liquorice,and added radix isatidis and liquorice to strengthen the effect of clearing away heat and detoxification and relieving poison carbuncle.In this study,the pharmacodynamics and acute toxicity tests of Qiqingbaidu powder were conducted,as well as the safety tests of the drug on target animals of pigs,and its clinical efficacy was observed,providing a theoretical basis for the future clinical application of Qiqingbaidu powder in porcine reproductive and respiratory syndrome.Methods: pharmacodynamics test: fever model was made by peptone to study the antipyretic effect of Qiqingbaidu powder.The method of auricle edema induced by xylene was used to study its anti-inflammatory effect.Staphylococcus aureus,escherichia coli and salmonella were selected as experimental bacteria,and the minimal inhibitory concentration(MIC)of Qiqingbaidu powder against these three common bacteria was determined by the test tube dilution method of broth.On the culture plate of 96 hole cells,the effect of Qizhui-Decoction on PRRSV was determined at the cell level.Results: the pharmacodynamics research shows that seven clear disease caused by dispersion can significantly inhibit peptone rabbit body temperature rise,can significantly reduce the mouse ear swelling caused by dimethylbenzene,who will take care of the common pathogenic bacteria e.coli bacterium,salmonella,staphylococcus aureus,all have certain inhibition,PRRSV has obvious inhibitory effect on cells.Conclusion: Qiqingbaidu powder has good antipyretic,anti-inflammatory,antibacterial and antiviral effects.更多还原