【作者】 刘刚；

【导师】 周海宇；

【作者基本信息】 兰州大学 ， 临床医学·外科学， 2019， 硕士

【摘要】 目的:本研究制备S-D大鼠的脊髓突触体,并对大鼠脊髓突触体中神经递质进行定性、定量分析研究。方法:本实验使用不连续性Percoll密度梯度离心的方法去提取大鼠脊髓的突触体,然后在透射电镜下观察大鼠脊髓突触体的形态及其结构。采用超高效液相色谱-质谱联用法对大鼠脊髓突触体中神经递质进行测定。结果:本研究制备的大鼠脊髓突触体,呈一形态结构完整且具有膜结构的椭圆形颗粒,其中包含有突触小泡、线粒体、细胞浆等。检测出大鼠脊髓突触体中含有多巴胺(DA)、去甲肾上腺素(NE)、3-甲氧酪胺(3-MT)、3-羟基-2-氨基苯甲酸(3-HAA)、5-羟色胺(5-HT)、高香草酸(HVA)、r-氨基丁酸(GABA)、3,4-二羟基苯乙酸(DOPAC)、5-羟基吲哚乙酸(5-HIAA)、色氨酸(TRP)、犬尿氨酸(KYN)、3-羟基犬尿氨酸(3-HK)等神经递质。结论:不连续性Percoll密度梯度离心法能制备出大鼠脊髓突触体,并可用液相色谱-质谱法检测出大鼠脊髓突触体中所含神经递质及其相关代谢产物,说明脊髓突触体具有生理活性。更多还原

【Abstract】 Objective: In this study,spinal synaptosomes of S-D rats were prepared,and qualitative and quantitative analysis of neurotransmitters in rat spinal synaptosomes was performed.Methods: In this experiment,discontinuous Percoll density gradient centrifugation was used to extract synaptosomes from rat spinal cord,and then the morphology and structure of rat spinal synaptosomes were observed under transmission electron microscope.Neurotransmitters in rat spinal synaptosomes were determined by ultra performance liquid chromatography-mass spectrometry.Results: The rat spinal synaptosomes prepared in this study is a morphologically intact and membrane-structured elliptical particle containing synaptic vesicles,mitochondria,cytoplasm and the like.Rat spinal synaptosomes were detected to contain dopamine(DA),norepinephrine(NE),3-methoxytyramine(3-MT),3-hydroxy-2-aminobenzoic acid(3-HAA),Serotonin(5-HT),high vanillic acid(HVA),r-aminobutyric acid(GABA),3,4-dihydroxyphenylacetic acid(DOPAC),5-hydroxyindoleacetic acid(5-HIAA),color Neurotransmitters such as tyrosine(TRP),kynurenine(KYN),and 3-hydroxykynurenine(3-HK).Conclusion: Rat spinal synaptosomes can be prepared by discontinuous Percoll density gradient centrifugation.The neurotransmitters and their related metabolites in rat spinal cord synaptosomes can be detected by liquid chromatography-mass spectrometry.The body is physiologically active.更多还原