【作者】 李敏；

【导师】 刘雁鸣；

【作者基本信息】 浙江大学 ， 口腔临床医学， 2018， 硕士

【摘要】 磷酸钙盐骨水泥材料,因其具有与骨类似的化学组分,在过去的骨修复研究中显出良好的生物相容性、骨传导性等性能,因此被广泛应用于临床。但是,一系列研究中显示出,较低的降解速率、较差的骨诱导性限制了其进一步的应用。在近年来的对生物活性陶瓷系统的研究中,通过包括制造技术在内的一系列技术的提高,对生物活性陶瓷各方面理化性能有了显著的改进。一些钙镁硅酸盐陶瓷具有高生物活性和生物可降解性,但其成骨潜能,尤其是生物力学表现还很少被证实。本研究探索了生物陶瓷墨水经三维打印技术制备的镁黄长石(Ca2MgSi207)多孔材料的成骨能力和力学性能,并与临床上使用的β-磷酸三钙多孔生物陶瓷进行了比较。这种三维打印的支架具有约250 μm大小的完全互连孔和50%以上的孔隙率,具有明显的抗压强度(72.4±8.5MPa),比β-磷酸三钙多孔生物陶瓷(12.5±3.4 MPa)高约7倍。进一步进行细胞培养试验后,其细胞增殖、碱性磷酸酶活力、成骨基因检测结果显示,镁黄长石组上的细胞增殖能力更强、碱性磷酸酶活力更高以及成骨基因mRNA表达明显增高。这些结果表明,三维打印镁黄长石多孔支架在骨组织再生方面具有良好的应用前景。更多还原

【Abstract】 Biomaterials of ca-silicate cements,due to its similar chemical composition to bone and good biocompatibility and osteoconductivity,have been applied in clinic as bone repair materials.However,the poor degradation and osteoinductivity limit its further application.With the development of a series technique,especially fabrication techniques,the physicochemical properties of this new bioceramic system has been significant improved.Herein,the osteogenesis capacity and mechanical evolution of the akermanite(Ca2MgSi2O7)porous materials manufactured by ceramic ink writing three-dimensional printing technique were investigated,in comparison with the clinically available β-tricalcium phosphate porous bioceramic.Such 3D printed akermanite scaffolds possess fully interconnected pores of～250 μm in size.And it porosity is over 50%with appreciable compressive strength(72.4±8.5MPa),that is 7-fold higher than that of the β-tricalcium phosphate porous bioceramics(12.5±3.4 MPa).Significant higher cell proliferation,alkaline phosphatase acitivity and mRNA expressions of osteogenic genes were detected in the akermanite group.These results suggest that the mechanically strong 3D printed akermanite scaffolds are promising for the bone tissue regeneration.更多还原