【作者】 周翔；

【导师】 顾宁；

【作者基本信息】 南京中医药大学 ， 中医内科学心血管病（专业学位）， 2017， 硕士

【摘要】 目的:探研导师经验方"化湿降浊方"对高尿酸血症模型大鼠的干预作用及部分作用机制,为中药复方的临床应用提供一定客观依据。方法:采用灌胃次黄嘌呤联合氧嗪酸钾腹腔注射[1]连续7d以复制高尿酸血症模型大鼠。同时予以别嘌呤醇为阳性药物对照,研究化湿降浊方(1.2g.ml-1)对各组血尿酸(UA)、肝脏黄嘌呤氧化酶(xanthine oxidase,XOD)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的影响;采用同样方法7d复制高尿酸血症模型大鼠,再予苯溴马隆为阳性药物对照,化湿降浊方(1.2g.ml-1)对各组灌胃7d,检测其对血尿酸(UA)、尿UA、尿素氮(BUN)、肌酐(Scr)的影响,并观察肾组织切片变化。运用SPSS17.0统计软件对上述数据进行分析,以P<0.05为差异有统计学意义。结果:1.与空白组对比,模型组大鼠血UA显著升高(P<0.01)。与模型组相比,中药组能有效抑制模型大鼠肝脏XOD的活性以达到降低血UA的作用,其差异具有显著性(P<0.01);中药组较别嘌呤醇组显著降低ALT、AST含量(P<0.05,P<0.01)。2.与空白组相比,苯溴马隆组、中药组均能有效促进模型大鼠尿UA排泄,达到降低血UA的作用,其差异具有显著性(P<0.01);中药组较模型组的血清Scr、BUN含量降低(P均<0.01);3.肾组织切片方面:化湿降浊组及苯溴马隆组对模型大鼠肾脏的损害程度均较模型组轻,其中化湿降浊组对模型大鼠肾间质损害度较苯溴马隆组轻,仅见轻度水肿伴少量尿酸结晶形成。结论:1.化湿降浊方能有效降低高尿酸血症模型大鼠的血尿酸水平。2.化湿降浊方能够有效抑制高尿酸血症模型大鼠肝脏黄嘌呤氧化酶的活性,并且不会引起ALT、AST的异常。3.化湿降浊方具有促进高尿酸血症模型大鼠尿酸排泄作用,有一定程度的肾功能保护作用。更多还原

【Abstract】 Objective:This research is reference to my tutor clinical experience prescription HuaShi Jiangzhuo Decoction intervention for hyperuricemia rats to clarify the efficacy and safety of the treatment of hyperuricemia.Provide objective basis for clinical application.Methods:By stomach feeding hypoxanthine combined with potassium oxonate by intraperitoneal injection last for 7days to produce hyperuricemia model rats.Meanwhile put allopurinol as positive control drug group for study influence of HuaShi Jiangzhuo Decoction(1.2g.ml-1)on serum UA、XOD、ALT、AST;Another use the same way continued 7days to replication hyperuricemia model rats,then give benzene bromine Malone as contro testl,HuaShi Jiangzhuo Decoction(1.2g.ml-1)for the oberservation group another 7days.To evaluate the effects of UA,urine UA,BUN,Scr,and changes of renal histopathology were detected.used spass 17.0 statistical software for the date from the above analysis,with P<0.05 for the difference was statistical significant.Results:1.Compare to the normal group,model group UA increased significantly(P<0.01).When Compared with model group,TCM group can effectively inhibit the activity of liver XOD to reduce the serum UA,the difference was significant(P<0.01);But TCM group decreased more significantly ALT,AST content than the allopurinol group(P<0.05,P<0.01).2.Compare to the Normal group,benzene bromine Malone group,TCM group can effectively promote urinary UA excretion,reduce UA,the difference was significant(P<0.01),TCM group significantly decreased the content of BUN and serum Scr than model group(P<0.01)3.Slices of kidney:huashi Jiangzhuo group and benzene bromine Malone group in the degree of kidney damage lessn than those in model group,the huashi Jiangzhuo group on renal injury contrast to benzene bromine Malone group,only less edema with a small amount of uric acid crystals formed.Conclusion:1.HuaShi Jiangzhuo Decoction can effectively lessen levels of serum uric in hyperuricemia rats;2.HuaShi Jiangzhuo Decoction can restrain the activity of liver xanthine oxidase,and never cause ALT,AST abnomarly changes in hyperuricemia rats;3.HuaShi Jiangzhuo Decoction can strengtn uric acid excretion,it means have certains of extents of protective impact on kidney function in hyperuricemia rats.更多还原