【作者】 王彬彬；

【导师】 杨志民；

【作者基本信息】 南京农业大学 ， 草业（专业学位）， 2015， 硕士

【摘要】 翦股颖是现代园林绿化和运动场建设最常用的冷季型草坪草之一。在我国长江中下游气候过渡地带,因其具有耐低修剪、绿色期长、质地细密等优良坪用性状而被广泛应用。但在该地区存在抗旱性差、不耐盐碱和越夏困难等问题,了解品种间性状差异和抗性差异,利用生长物质调控耐盐性,对城市园林和运动场正确选择优良品种,改善盐碱地翦股颖草坪质量具有实际意义。本试验以近年来从国外引进的翦股颖品种为研究对象,从田间适应性评价,抗旱耐盐性筛选,以及盐胁迫下三种外源物质对匍匐翦股颖的生理调控三个方面,通过田间观测和盆栽试验,对不同翦股颖品种的坪用性状、抗旱耐盐性作出评价,同时探讨叶面喷施K2SO4、抗坏血酸、甜菜碱三种小分子外源物质对盐胁迫下匍匐翦股颖的缓解效应。具体研究内容和结论如下:对10个翦股颖品种进行田间NTEP评价,结果表明:BT020(Tiger)、BT010(Penncross)的TQ、颜色显著优于其他品种;BT005(L-93)、BT020的盖度显著优于其他品种;BT017(Agrostis scabra)的TQ、盖度、颜色显著劣于其他品种。但在质地上,BT017得分最高,BT010、BT018(CENTury)得分较低,与其它品种均表现出显著性差异。采用盆栽试验对8个翦股颖品种进行抗旱耐盐性评价,结果表明:干旱和盐胁迫处理下均发生叶片枯黄、生长缓滞、草坪质量下降的现象。干旱胁迫下BT005(L-93)的TQ显著优于其它品种;盐胁迫下BT017(Agrostis scabra)、BT021(Xinye)的TQ显著劣于其它品种,其它6个品种间差异不显著。其中BT017的抗旱、抗盐能力均是最差,与其他品种均表现出显著性差异。盐胁迫导致"L-93"的草坪质量降低、相对含水量降低、相对电导率升高、细胞膜稳定性降低、抗氧化酶的活性降低。盐胁迫条件下叶面喷施K2S04或抗坏血酸(Asb),可显著提高其相对含水量,降低相对电导率,降低叶片Na+含量的积累,提高叶片K+含量,从而降低盐胁迫下Na+/K+值,还能在一定程度上增强"L-93"细胞的抗氧化性,显著提高过氧化氢酶(CAT)的活性,降低丙二醛(MDA)的含量。更多还原

【Abstract】 Agrostis is one of the most common type of cold season grass in the modern landscaping and sports venues construction.In China climate transition zone as the middle and lower reaches of the Yangtze river.It is widely used because of its low resistance trim,long green period and fine texture.But there are poor drought resistance,Intolerance with salinity and summering difficult issues in this region.It will have practical significance when we learn the different resistant between different Traits,and make the right choice of good varieties of urban garden and playground.Moreover,Using growth substances control salt resistance can improve bentgrass turf quality in salt area.With bentgrass imported from abroad in recent years as the research object in this test.This article do the research from three aspects,as the evaluation of adaptability in field,and screening with drought resistance and salt resistance,as well as physiological regulation of creeping bentgrass with three kinds of exogenous substances under salt stress.We will do the field observation and pot experiment,to evaluate different bentgrasses,Discussion the effect of exogenous substance on creeping bentgrass under salt stress,when spraying K2SO4,Ascorbic acid,Glycine betaine to the leaf.The specific research contents and conclusions are as follows:We made 10 varieties of bentgrass for NTEP evaluation in the field,the results indicate:BT020,BTO10’s TQ significantly superior to other varieties,BT005,BT020’s coverage was significantly better than other varieties,TQ,coverage and color of BT017 are significantly worse than other varieties,But on the texture,BT017 has highest score,BT010,BT018 have low scores,They all shown significant differences with other species.The pot experiment of 8 varieties of bentgrass evaluation of drought resistance and salt resistance,the results indicate:It bring about the phenomenon of turf quality decline,the leaf withered and yellow,growth slowing under drought and salt stress.BT005 got the highest score under drought stress,significantly better than BT004,BT021,BT017.the TQ of BT017 and BT021 are significantly worse than other varieties under salt stress.BT017 are the worst under drought resistance and salt resistance,Showed significant difference with other varieties.Salt stress results in the decrease of L-93’s turf quality,the decrease of Relative Water Content,and increase the Electrolyte leakage,decrease the cell membrane stability,as well as made the decrease of antioxidant enzymes activity.Spraying on the leaf with K2SO4 or Ascorbic acid under salt stress,Can significantly improve the relative water content,decrease the Electrolyte leakage,and lower leaf content of Na+ accumulation,improve leaf content of K+.Moreover,It can enhance L-93’s cell oxidation resistance to a certain extent.Significantly increase the activity of Catalase,and lower the Malondialdehyde content.更多还原