【作者】 张荣；

【导师】 刘丽梅；

【作者基本信息】 上海交通大学 ， 内科学（内分泌与代谢病学）， 2015， 硕士

【摘要】 目的探讨肾胺酶基因(RNLS)rs2576178变异是否与合并微量白蛋白尿的2型糖尿病肾病(DN)患者高血压发病相关。方法以上海地区汉族226例合并微量白蛋白尿的2型糖尿病肾病患者(DN组)和251例无糖尿病、无肾脏疾病的健康对照者(Cont组)作为研究对象,DN组分为高血压组DN-HTN(+)169例和正常血压组DN-HTN(-)56例进行分析。采用Taqman探针方法,检测以上研究对象RNLS基因rs2576178(G/A)基因型,比较组间基因型、等位基因频率及临床变量间的差异。结果1)DN组和Cont组rs2576178(G/A)基因型GG、GA、AA频率分布符合Hardy-Weinberg平衡定律(P>0.05 for each)。与Cont组相比,DN组AA基因型和A等位基因频率显著降低(P<0.05 for each);2)应用非条件Logistic回归分析对全组(DN组+Cont组)校正性别、年龄、BMI后,rs2576178AA基因型仍与高血压显著相关,OR值为0.48(95%CI 0.28~0.81);3)与DN-HTN(-)组相比,DN-HTN(+)组GG+GA基因型和G等位基因频率显著增加(P<0.05 for each)。对DN组校正性别、发病年龄、BMI后,rs2576178AA基因型亦与高血压显著相关,OR值为0.37(0.17~0.79)。结论RNLS基因rs2576178AA基因型可能具有防止合并微量白蛋白尿的2型糖尿病肾病患者高血压发生的作用。更多还原

【Abstract】 Objective To investigate if the rs2576178 in renalase gene(RNLS) is associated with hypertension in type 2 diabetic nephropathy with micro-albuminuria patients.Methods The study population consists of unrelated 226 type 2 diabetic nephropathy with microalbuminuria patients(DN group) and 251 non-diabetes without nephropathy control subjects(Cont group). The DN group was further divided into DN-HTN(+) group, n=169 and DN-HTN(-) group, n=56 according to if the hypertension exists. Genotypic and allelic frequencies of rs2576178 as well as clinical characteristics were compared among groups, Taqman PCR assay was performed for the genotyping of all subjects.Results 1)Three genotypes(GG, GA and AA) of rs2576178(G/A) were detected. The genotype distribution of rs2576178(G/A) was in consistent with Hardy- Weinberg equilibrium. AA genotypic and A allelic frequencies were decreased in DN group when compared with Cont group(P<0.05 for each). 2)AA genotype of rs2576178 is significantly associated with preventing hypertension in all subjects, by multiple logistic regression analysis, adjusted for sex, age, and BMI with OR(95% CI) of 0.48(0.28~0.81). 3)Compared with DN-HTN(-) group, GG genotypic and G allelic frequencies were significantly increased(P<0.05 for each). Adjusted for sex, onsetage of diabetes and BMI, AA genotype of rs2576178 is significantly associated with preventing hypertension in DN patients with OR of 0.37(0.17~0.80) by multiple logistic regression analysis.Conclusion AA genotype carriers of rs2576178 in RNLS may decrease the risk of hypertension in microalbuminuria of type 2 diabetic nephropathy when adjusted for sex, onset-age and BMI.更多还原