【作者】 杨虎；

【导师】 薛蔚；

【作者基本信息】 上海交通大学 ， 外科学 泌尿外科专业， 2015， 硕士

【摘要】 目的:研究AngII及替米沙坦在前列腺癌进展中所起的作用及其可能机制。方法:采用Western印迹法、q RT-PCR法检测AT-1在各细胞系(LNCaP、DU145、PC3)中的表达情况。组织芯片检测AT-1在不同组织类型的前列腺组织(正常前列腺、前列腺增生组织、前列腺癌组织)中的表达情况。CCK-8法检测AngII、替米沙坦(AT-1受体拮抗剂)对癌细胞增殖的影响。Transwell法检测AngII、替米沙坦对癌细胞迁移及侵袭的影响。Western印迹法检测AngII、替米沙坦对癌细胞侵袭相关的MMP-2、MMP-9表达的作用。结果:AT-1在三个前列腺癌细胞系中均有表达,表达程度由高到低依次为DU145、PC3及LNCaP。组织芯片结果提示:随着前列腺癌恶性程度增加,AT-1表达增加。AngII可促进DU145增殖、迁移及侵袭,替米沙坦可抑制该作用。Ang II可促进DU145侵袭相关的MMP-2、MMP-9表达,替米沙坦可抑制该作用。结论:AngII及替米沙坦在前列腺癌进展中起一定作用。在前列腺癌的治疗中替米沙坦可能发挥一定的作用,其中机制有待进一步研究。更多还原

【Abstract】 Objective: Study the effect of Ang II and telmisartan playing in the progression of prostate cancer and its possible mechanism.Methods: Using Western blotting and q RT-PCR method to detect AT-1 expression in LNCa P, DU145 and PC3 cell lines.Using tissue microarray to detect AT-1 expression in different histological types of prostate tissues(normal prostate, prostate hyperplasia, prostate cancer). Detect the effect of Ang II and telmisartan(AT-1 receptor antagonist) on proliferation of cancer cells by CCK-8 method. Using transwell method to detect the effect of Ang II and telmisartan on migration and invasion of cancer cells. Detect the effect of Ang II and telmisartan on MMP-2, MMP-9 expression of cancer cells via Western blotting, which is related on invasion.Results: AT-1 is expressed in the three prostate cancer cell.From high to low of the degree of expression is DU145,PC3 and LNCa P. The results of tissue microarray suggest AT-1 expression increase with the development of prostate cancer. Ang II can promote the proliferation, migration and invasion of DU145, and telmisartan can inhibit it. Ang II can enhance the expression of MMP-2 of DU145 associated with invasion and telmisartan can inhibit the effect.But they have no obvious effect on the expression of MMP-9.Conclusion: Ang II and telmisartan play a certain role in prostate cancer progression. In the treatment of prostate cancer,telmisartan may play a certain role, which needs further study.更多还原