【作者】 秦桂芳；

【导师】 丁良；

【作者基本信息】 河北大学 ， 药理学， 2016， 硕士

【摘要】 目的:探讨猪苓汤干预对卡介苗(Bovisbacilli Calmette Guerin,BCG)预防N-甲基亚硝基脲(N-methyl-1N-nitrosourea,MNU)致大鼠膀胱癌的作用。方法:建立MNU致大鼠膀胱癌模型,并用猪苓汤按照0.75g/kg1.5g/kg、3g/kg的剂量给大鼠灌胃,进行干预。实验过程中,记录大鼠体重、进食量变化。实验结束后,处死大鼠,计算大鼠膀胱、胸腺、脾脏的脏器指数,剥离膀胱进行病理学检查,用ELISA检测血清TNF-α和ICAM-1水平。结果:1.猪苓汤各组大鼠体重与模型组比较,无显著差异,各组进食量无统计学意义。2.猪苓汤中、低组可以显著升高大鼠膀胱指数(P<0.05),但对胸腺及脾脏未见明显影响(P>0.05);3.各实验组大鼠膀胱病理组织学评分有升高的趋势,其治疗效果不及卡介苗组对照组;4.猪苓汤可下调由于灌注BCG引起的TNF-α、ICAM-1浓度升高。结论:猪苓汤可降低大鼠血清中TNF-α、ICAM-1的浓度,导致BCG抑制膀胱癌的疗效下降。更多还原

【Abstract】 Objective: To explore the effect of Zhuling decoction for Bovisbacilli Calmette Guerin(BCG) on bladder tumor induced by N-methyl-N-nitrosourea(MNU) in rats. Methods: Bladder carcinoma rats model was made by intravesical instillation of MNU. Meanwhile, rats were orally administered with Zhuling Decoction(0.75g/kg, 1.5g/kg, 3g/kg) regularly and intravesical administration of BCG on time. Animals were sacrificed after the experiment. The bladder, thymus and spleen indexes of all groups were calculated. Levels of serum Tumor necrosis factor-α(TNF-α) and Intercellular cell adhesion molecule-1(ICAM-1) were assayed by ELISA, and histological examination was done. Results: 1. Compared to the model rats group, there was no significant difference that the rats weigh given Zhuling decoction and food intake that all rats group. 2. The groups of middle and small dose of Zhuling decoction could reduce bladder index of rats with bladder tumor(P<0.05), but thymus index and spleen index have no significant difference(P>0.05). 3. The tumor pathological grades in Zhuling decoction groups were also descended, and the outcomes were not better than those of BCG group. 4. The level of serum TNF-α,ICAM-1 in Zhuling decoction group was decreased after treatment. Conclusion: Our findings convincingly showed that Zhuling decoction and its decomposed recipes inhibited the antitumor effect of BCG through declining the level of TNF-α and ICAM-1.更多还原