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何首乌饮对自然衰老大鼠肾脏CD26表达的影响
Effect of Heshouwuyin on CD26 Expression in The Kidneys of Aging Rats
【作者】 赵荣慧；
【导师】 牛嗣云；
【作者基本信息】 河北大学 ， 药理学， 2015， 硕士
【摘要】 目的:观察CD26在自然衰老大鼠肾组织中的表达,探讨何首乌饮对自然衰老大鼠肾组织CD26表达的影响。方法:选用Wistar大鼠随机分组:青年对照组(12月龄)大鼠,自然衰老组(18月龄)大鼠,何首乌饮干预组(18月龄)大鼠,每组10只。自然衰老组生理盐水灌胃;何首乌饮干预组何首乌饮灌胃60天。采用HE染色显微镜下观察肾组织形态学的变化;免疫组化法和蛋白印记法观察CD26在肾脏组织表达量的变化。结果:与青年对照组相比,CD26在自然衰老组大鼠肾脏的表达明显增强(P<0.01);何首乌饮干预组肾组织肾小球数目明显高于自然衰老组,与青年对照组接近(P<0.01)。CD26主要表达在肾间质细胞细胞质。CD26在自然衰老组大鼠肾脏的表达较青年对照组明显增强(P<0.01)。何首乌饮干预组CD26表达明显低于自然衰老组(P<0.01)。结论:何首乌饮能改善自然衰老大鼠肾脏的形态学结构,并减弱自然衰老大鼠肾脏CD26的表达,具有延缓衰老作用。
【Abstract】 Objective:To investigate the effect of Heshouwuyin on CD26 expression in the kidney tissues of natural aging rats.Methods:Wistar rats were randomly divided into three groups:natural young group(NY group, 12 months),aging model group(AG group,18mongths)and Heshouwuyin-treated aging group(HT group,18months),each group has ten rats.AG wistar rats were fed with 0.9% NaCl.HT wistar rats were fed with Heshouwuyin for sixty days. The slices of kidney were made by histological method and stained by HE staining.The morphological changes of kidney were observed under microscope and quantitatively analyzed.The expression of CD26 in kidney was examined by using immunohistochemistry and western。Results : Compared with NY group, CD26 were significantly increased in AG group(P<0.01).The kidney glomerulus number of rats in HT group was higher than in AG group and approach NY rats(P < 0.01).CD26 in renal tissue was mainly expressed in interstitial cell of renal interstitium.CD26 expression in AG group was significantly higher than that in NY group(P<0.01);In HT group, CD26 expression was lower than that in AG group(P<0.01).Conclusions: CD26 is related with aging nephropathy.Heshouwuyin maybe reduce the expression of CD26 in renal interstitium,thus protecting the kidney function and delay renal senility of rats.