【作者】 刘明；

【导师】 张庆祥；

【作者基本信息】 山东中医药大学 ， 中医基础理论， 2016， 硕士

【摘要】 目的:探讨大鼠支气管哮喘寒饮蕴肺证气道炎症的机理,研究温阳化饮方作用机制。方法:采用卵蛋白致敏加冰箱冷冻、冰水饲养、温水游泳等方法,建立支气管哮喘寒饮蕴肺证大鼠模型,分别用1,25(OH)2D3、小青龙汤、温阳化饮方和六君子汤进行干预,观察相关检测指标。结果:造模较为成功,模型组大鼠的一般行为学表现与支气管哮喘寒饮蕴肺证的临床表现基本相符,微观指标的变化也证实造模较为成功;治疗组的四种药物对上述各项指标都有不同程度的改善,其中温阳化饮方疗效最佳;小青龙汤组和温阳化饮方组药物均对大鼠血液1,25(OH)2D3含量有明显促进作用,其中,温阳化饮方组作用最显著,而1,25(OH)2D3组和六君子汤组对大鼠血液1,25(OH)2D3含量促进作用不明显。结论:针对支气管哮喘寒饮蕴肺证,温阳化饮方确有较好疗效。同时发现温阳化饮方能提升支气管哮喘寒饮蕴肺证大鼠血液中1,25(OH)2D3的含量,提示温阳化饮方疗效机制可能是通过改善大鼠血液中1,25(OH)2D3的含量,进而改善气道炎症和气道重塑。说明温阳化饮方的温补肺阳,温化寒饮及改善肺通气的作用,与其调控体内1,25(OH)2D3的含量有关。更多还原

【Abstract】 Objectives: The thesis aims to probe into the airway inflammatory pathogenesis of rats of bronchial asthma with syndrome of cold fluid retained in lung and discuss the mechanism of warming yang and resolving fluid retention.Methods: Methods of ovalbumin sensitization, refrigeration, raising in ice water,swimming in warm water are employed to make a rat model of bronchial asthma with syndrome of cold-fluid retained in lung. Then, 1,25(OH)2D3, Xiaoqinglong Decoction,formula of warming yang and resolving fluid retention, and Liujunzi Decoction are adopted in experiment respectively to observe the test index.Results: The rat model is successfully made because the behaviors of rats of experiment group are consistant with clinical manifestations of bronchial asthma with syndrome of cold-fluid retained in lung, which is also confirmed by micro index. The four above mentioned formulas can improve the syndrome, among which formula of warming yang and resolving fluid retention is the most effective in terms of efficacy. Xiaoqinglong Decoction and formula of warming yang and resolving fluid retention can increase the content of 1,25(OH)2D3 and the latter is better. However, Liujunzi Decoction is not effective.Conclusion: Formula of warming yang and resolving fluid retention is most effective in treating bronchial asthma with syndrome of cold fluid retained in lung, which can also increase the content of 1,25(OH)2D3 of rat model. It indicates that the treatment mechanism of formula of warming yang and resolving fluid retention acts by increasing the content of1,25(OH)2D3, and by reliving the inflammation of airway. Based on our study, we come to the concusion that formula of warming yang and resolving fluid retention can warm and tonify lung yang, revolve cold fluid, and improve lung qi, which are related with thecontent of 1,25(OH)2D3.更多还原