【作者】 李坚；

【导师】 周凯；

【作者基本信息】 南昌大学 ， 外科学， 2016， 硕士

【摘要】 目的:通过检测CIP2A与c-Myc在乳腺纤维腺瘤、非浸润性乳腺癌、浸润性乳腺癌中的表达情况,明确不同阶段乳腺肿瘤组织中CIP2A和c-Myc各自的表达水平以及二者表达的相关性,初步的探讨两因子在乳腺癌发生发展中的作用。方法:选取纤维腺瘤、非浸润性乳腺癌、浸润性乳腺癌标本各40份。采用实时定量逆转录聚合酶反应方法检测40例乳腺纤维腺瘤,40例乳腺原位癌和40例浸润性乳腺癌中的CIP2AmRNA的表达情况,并比较它们的差异。采用Westernblot检测40例乳腺纤维腺瘤,40例乳腺原位癌和40例浸润性乳腺癌中的CIP2A及c-Myc蛋白的表达情况,比较它们的差异。用免疫组织化学方法检测40例乳腺纤维腺瘤,40例乳腺原位癌和40例浸润性乳腺癌中CIP2A及c-Myc蛋白的表达情况,比较它们的差异;并分析CIP2A与c-Myc表达的相关性。所有数据均通过SPSS17.0统计软件进行t检验、单因素方差分析、秩和检验及Spearman等级相关分析,以P<0.05表示差异有统计学意义。结果:1.CIP2AmRNA在乳腺纤维腺瘤、非浸润性乳腺癌、浸润性乳腺癌中的表达依次升高,差异有统计学意义(P<0.05);CIP2AmRNA在浸润性乳腺癌中过表达。2.CIP2A及c-Myc蛋白在乳腺纤维腺瘤、非浸润性乳腺癌、浸润性乳腺癌组织中表达的强度依次升高,差异有统计学意义(Westernblot检验行单因素方差分析P<0.05;免疫组织化学方法检测行秩和检验P<0.05);CIP2A及c-Myc蛋白在浸润性乳腺癌组织中过表达。3.Spearman等级相关分析显示在非浸润性乳腺癌和浸润性乳腺癌中CIP2A与c-Myc表达的强度呈正相关(浸润性乳腺癌r=0.536、P<0.05;非浸润性乳腺癌r=0.462,P<0.05)。结论:CIP2A与c-Myc在浸润性乳腺癌中过表达;二者在乳腺纤维腺瘤、非浸润性乳腺癌、浸润性乳腺癌中的表达程度依次升高;两者的表达呈正相关;CIP2A可能通过稳定c-Myc蛋白参与乳腺癌的发生和发展。更多还原

【Abstract】 Objective:The present study was aimed to investigate the expression level of CIP2 A and c-Myc and to definite their relationship in different stages of breast tumor by detect the expression of CIP2 A and c-Myc in breast fibroadenoma, noninvasive breast cancer and invasive breast cancer. Thereby, to primary explore the role of these two factors in the occurrence and development of breast cancer. Methods:In this study,we randomly select the samples of breast fibroadenoma, noninvasive breast cancer and invasive breast cancer, all three groups contain 40 cases. The expression of CIP2 A mRNA in each group samples were measured by Reverse transcription polymerase chain reaction(RT-PCR)and agarose gel electrophoresis, and then compared the differences between them.In addition, western blot and immunohistochemistry were applied to detect the expression of CIP2 A and c-Myc protein in each group samples, then the differences was compared between them. At last, the correlation of the expression of CIP2 A and c-Myc in breast tumor was analyzed. All data were evaluated using t-test, One-way ANOVA analysis, Wilcoxon rank sum test and Spearman rank correlation analysis which were performed by statistics software SPSS17.0. A P value <0.05 was considered to be statistically significant. Results:1.The relative expression level of CIP2 A mRNA was gradually increased in breast fibroadenoma, noninvasive breast cancer and invasive breast cancer, the differences was statistically significant(P<0.05). Moreover, CIP2 A mRNA was overexpressed in invasive breast cancer.2.From breast fibroadenoma, noninvasive breast cancer to invasive breast cancer, the expression intensity of CIP2 A and c-Myc protein were gradually increased, the differences was statistically significant(the results of western blot were evaluated by One-way ANOVA analysis, P<0.05; the results of immunohistochemistry were analyzed by Wilcoxon rank sum test, P<0.05). Furthermore, the protein of CIP2 A and c-Myc were overexpressed in invasive breast cancer.3.The Spearman rank correlation analysis showed that the expression intensity of CIP2 A and c-Myc was positive correlated in noninvasive breast cancer(r=0.462, P<0.05) and invasive breast cancer(r=0.536, P<0.05). Conclusions:We conclude that the expression intensity of CIP2 A and c-Myc were gradually increased in breast fibroadenoma, noninvasive breast cancer and invasive breast cancer, and these two proteins were overexpressed in invasive breast cancer. The character of CIP2 A positive related with c-Myc implying that CIP2 A may participate in the occurrence and progression of breast cancer by stabilizes c-Myc.更多还原