【作者】 李浩；

【导师】 宋文龙；

【作者基本信息】 吉林大学 ， 高分子化学与物理， 2016， 硕士

【摘要】 随着社会的进步发展,人们出于对治愈疾病、提高生活水平的良好意愿,对生物材料提出了越来越高的要求,尤其是高分子生物材料因普遍具有许多金属和无机材料所无法取代的优点而获得迅速的发展。高分子水凝胶材料具有较高的含水率、良好的生物相容性、低摩擦系数,被广泛应用于组织工程支架、药物传输系统等方向。对于可植入医用高分子水凝胶材料,在要求其与原组织力学性能相匹配的同时,还需避免可能残余的病变细胞在材料表面的粘附增殖所引起的病变。因此,综合调控材料硬度和表面对细胞增殖有抑制作用的超浸润特性的协同作用,为设计实用可植入材料提供了新的思路。本论文应用具有良好生物相容性的高分子材料,结合模板法和调节交联剂配比,首先实现对材料机械性能和表面结构的控制;在其表面构建微纳米复合结构实现膜表面的超浸润特性;通过电镜、谱学等手段,在微纳尺度和分子水平上研究它们的协同作用对肝癌细胞的粘附等生理活动的影响,总结细胞和材料表面相互作用的规律性,并优化细胞的粘附及分化行为,为设计新型的组织工程支架材料提供实验依据。更多还原

【Abstract】 With the progress and development of the society, the expectation and requirements of human beings for biological materials are increasing due to the good willing of curing disease and improving living standards. In particular,polymer biomaterials which have the irreplaceable advantages over many metallic and inorganic materials, has made a rapid progress. Polymer hydrogel materials have been widely applied in the field of tissue engineering scaffolds and drug delivery systems because of its high moisture content, favorable biocompatibility and low friction coefficient. As for implantable medical polymer hydrogel materials, not only should they match the mechanical property with the original tissues but also avoid the lesion caused by adhesion and proliferation of possible residual diseased cells on the material surface. Thus,the synergistic effect of surface comprehensive regulation in material hardness and ultra-wetting characteristics which inhibit cell proliferation offers a new direction for designing practical implantable materials.This paper achieves the control of the mechanical property and surface structure for polymer materials with good biocompatibility by template method and adjusting the ratio of crosslinking agent. Additionally, we implement their ultra-wetting characteristics via constructing micro/nano composite structure on surface of the membrane. We have successfully investigated the impacts ofsynergy on the physical activities such as the adhesion of hepatic carcinoma cells etc. Then we summarize the regularity of interaction between cells and material surfaces and further optimize the adhesion and differentiation of cells.To conclude, we hope our work can provide some valuable guidance for further materials design in the applicaiton of tissue engineering scaffold.更多还原