股形态发生蛋白2壳聚糖纳米缓释载体的制备及性能测定

【作者】 刘峰；

【导师】 孙健；

【作者基本信息】 青岛大学 ， 口腔医学， 2015， 硕士

【摘要】 目的:制备负载骨形态发生蛋白-2(BMP-2)的壳聚糖纳米微球,测定纳米球的粒径、zeta电位、形态、降解和药物释放性能,以探讨负载骨形态发生蛋白-2(BMP-2)的壳聚糖纳米球作为缓释载体的可行性。方法:以壳聚糖(Chitosan)和三聚磷酸钠(Tripolyphosphate)为原料,采用离子交联的方法制备壳聚糖纳米球。应用纳米粒度分析仪及zeta电位测定仪检测微球粒径、分布及zeta电位,透视电镜测定其表面形态。Elisa法测定包封率,载药率及药物释放率。采用SPSS19.0统计学软件对数据进行统计分析。结果:本实验制备的BMP-2壳聚糖纳米球,成球性好,球形态较规则,分散较均匀,无团聚现象,表面较光滑。平均粒径150.85 nm,分散指数PI=0.37,Zeta电位+35.42m V。包封率(68.24±3.83)%,载药率(56.83±2.26)%,体外释药试验BMP-2可以从壳聚糖纳米微球中缓慢释放,释放行为符合双相动力学规律,释放过程可达30d。结论:本研究制备的负载骨形态发生蛋白-2(BMP-2)的壳聚糖纳米微球可持续缓释BMP-2。释药规律符合双相动力学,并具有良好的降解稳定性,能够很好地满足构建组织工程骨缓释载体的要求。更多还原

【Abstract】 Purpose:The aim of this work was to prepare chitosan nanospheres for load BMP-2and to evaluate its size, Zeta potential,appearance,degradation and release characteristic in vitro, and then to evaluate its feasibility as a carrier for sustained release of BMP-2.Method : The BMP-2 loaded chitosan nanospheres were prepared using ionic crosslinking method with tripolyphosphate(TPP) and chitosan. Transmission electron microscope was used to evaluate the morphological properties, and laser particle size analyzer was used to analyze particle size,Zeta potential and distribution. Lysozyme degradation experiment was performed to assess the biodegradation behavior. ELISA assay was used to determine the loading efficiency, encapsulation efficiency and in vitro drug release kinetics.The data were analyzed by SPSS software according to statistics method.Results:The BMP-2 loaded chitosan nanospheres was spherical in shape, smooth on surface and uniform dispersion without aggregation. The mean diameter was 150.85 nm.The Dispersion Indexis was 0.37, Zeta potential was +35.42 mV. The average loading efficiency and encapsulation efficiency were(56.83 ± 2.26)% and(68.24 ± 3.83)%,respectively. The release experiment in vitro showed that the releasing property of BMP-2loaded chitosan nanospheres was consistent with two phase kinetic regulation and BMP-2 was controlled to release from the chitosan nanospheres over 30 days.Conclusions : The BMP-2 loaded chitosan nanospheres prepared by ioniccrosslinking method was successfully prepared and shows a good controlled release property. It provide the basis for further application of bone tissue engineering.Project supported by the Shandong Province Natural Science Foundation of China(Grant No.ZR2012HM069)更多还原