【作者】 陈琳；

【导师】 宋粉云；

【作者基本信息】 广东药学院 ， 药物分析学， 2015， 硕士

【摘要】 “药对”通常由两味相对固定的中药组成,是中药复方中最基本、最简单和最常见的形式?对“药对”配伍的研究是复方配伍规律研究的基础和重要切入点之一?药对广藿香-广陈皮两者配伍,广藿香化湿健脾、发表解暑,和中化湿,理气止呕?辛温但不燥热,气芳香但不燥烈,既能发表解暑,辟秽化浊,又能温中快气醒脾胃,专治脾胃湿浊呕逆?广陈皮性温和,燥而不烈,辛散苦降,具有理气健脾,燥湿化痰调中的功效。二药相合使用,广陈皮可助广藿香辟秽化浊,止呕止泻之力?治疗霍乱吐泻、外感暑湿或湿浊内蕴所致的脘闷痞满,食少纳呆,吐泻并作等症?目前主要围绕其药理作用做研究,缺乏对其配伍后的药效影响的物质研究,不能科学的阐述其配伍作用的机制,因此,本文应用GC-MS技术,对其挥发性成分进行研究。本学位论文的主要研究工作如下:一、基于响应面法优化广藿香-广陈皮药对的挥发油提取工艺采用响应面法对广藿香-广陈皮药对挥发油的提取工艺进行考察,最终确定药材粒度60目,液料比为10倍,提取时间5h,在此工艺条件下,药对挥发油的平均提取率为3.40%。二、广藿香-广陈皮药对挥发油的体外指纹谱研究对其进行精密度、重复性与稳定性的方法学考察后,分别运用GC-MS结合保留指数对药对广藿香-广陈皮、单味药广藿香和广陈皮的定性分析出134,121,85个成分,分别占挥发性成分总含量的84.01%,82.80%,78.79%。药对广藿香-广陈皮与广藿香的共有成分为59个,与广陈皮的共有成分有55个,三者的共有成分为23个,在广藿香-广陈皮药对中相对含量较高的挥发性成分在两种单味药中均可测出,但含量有所不同。广藿香-广陈皮药对挥发油的成分在种类上几乎是单味药材广藿香、广陈皮二者的加和。三、广藿香-广陈皮药对挥发油体内代谢谱的研究采用血清药物化学手段对广藿香-广陈皮挥发油的入血成分进行了研究,通过GC-MS的分析并结合保留指数的鉴定,确定了20个原型入血成分,2个代谢产物,明确了广藿香-广陈皮挥发油的药效物质基础,为进一步开展广藿香-广陈皮挥发油的作用机制研究打下了良好的基础。四、广藿香-广陈皮药对不同配伍比例对主要成分溶出的初步探讨对不同配比的广藿香与广陈皮配伍提取的挥发油中主要化学成分的变化规律进行探讨。实验发现,广藿香、广陈皮配伍后形成的挥发油中,其中主要成分的量均呈现不同程度的变化,其中包括一些已经报道有药理作用的成分,如d-柠檬烯、广藿香醇等,这些变化的规律为广藿香-广陈皮药对的配伍研究提供了科学的数据。本次研究不仅丰富了该药对的药效物质基础,也为含有该药对的复方制剂的研究提供依据,为其临床治疗提供一定的科学依据。更多还原

【Abstract】 Pogostemonis Herba(PH) is a common used Traditional Chinese Medicinal herb.It is used to remove dampness, relieve summer-heat, stop vomiting and stimulate the appetite. Pericarrium Citri Retieultae(PCR) has the effect managing gas tonifying spleen and the eliminating dampness reducing phlegm. The compatibility of PH and PCR increased the efficacy of preventing vomiting, alleviate diarrhea and removing dampness, have been used to malaria of cholera, exogenous damp, or stuffy gastral cavity ruffian is full, eat less indigestion which caused by phlegm-damp.Presently, most attentions are paid to its pharmacological effects. For the purpose of making full use of the medicinal herb, several parts from herbal pair are studied.First, investigate the extraction condition of volatile oil distilled from herbal pair(HP) of Pogostemonis Herba(PH) and Pericarrium Citri Retieultae(PCR). Based on single factor experiment, with the theory of Response Surface Methodology,optimization of steam distillation of volatile oil extraction technology. The optimal conditions of extraction process were as follows: sift 60 mesh sieve, 10:1 for water:powder and 5h for extraction.Second, the volatile oil of the herbal pair, Pogostemonis Herba(PH) and Pericarrium Citri Retieultae(PCR) were analyzed by gas chromatography-mass spectrometry(GC-MS), with automated mass spectral deconvolution and identification system(AMDIS) based on the temperature-programmed retention in dex(PTRI). Comparing the difference between chromatographys to identify the main chemical constituents, and the relative concentration of each component was determined by peak area normalization.The results show that there are 85, 121 and134 kinds of constituents of the essential oils being identified respectively from single herb PCR, PH and PH-PCR, and the relative amount accounts for 78.79%, 82.80%and 84.01% of the total relative contents in order. There are 23 common volatile constitutions in single herb and PH-PCR, while there are 55 and 59 common volatile constitutions in the single herb PCR and PH respectively. The compatibility of the drug pair has an effect on components and contents in the volatile oil of PH and PCR.Their relative kinds and amount in PH-PCR are not equal to the two single herbs,whose reaction mechanism in PH-PCR should be studied further.Third, based on the theory of serum Pharmaceutical Chemistry, we established the analysis method for volatile oil from the herbal pair by using GC-MS. By the comparing the chromatogram of volatile oil from the herbal pair, blank SD rat’s plasma and dosed SD rat’s plasma, 20 original compounds from volatile oil and 2metabolites were identified.Last, to analyze the main component in volatile oil of PH-PCR with different compatibility proportions, and study on the content of the main compounds in volatile oil of PH-PCR compatibility of different compatibility proportions and try to explain the mechanism of this compatibility. In the found, the volume of main component,such as β-elemene, patchouli alcohol, display different degree of variations, some of them have been reported to have pharmacological effects.The chemical fingerprint in vitro and metabolic profile in vivo of herbal pair decoction were established in our study. Meanwhile, some chemical compounds especially flavonoids were isolated and identified by modern analysis techniques. A systemic method of isolation, purification, identification and screening of pharmacodynamic substance for chemical constituents in the TCM( traditional Chinese medicine)was established, which could supply a new thinking and scientific basis for the overall quality control of TCM.更多还原