【作者】 张波；

【导师】 时茜；

【作者基本信息】 温州大学 ， 无机化学， 2015， 硕士

【摘要】 根据沃森-克里克碱基互补配对原则,利用DNA模板将反应物进行选择性组合,通过分步法成功合成了一系列不对称席夫碱金属配合物[Zn2LnenCl2],(L1=D3-4M-D4-4M、L2=D3-4M-D4-4H、L3=D3-4M-D4-4T、L4=D3-4M-D4-4Cl、D3=C182H236O108N71P18、D4=C182H239O111N66P18、4M=4-甲基-2,6-二甲酰基苯酚、4H=2,6-二甲基苯酚、4T=4-叔丁基-2,6-二甲酰基苯酚、4Cl=4-氯-2,6-二甲酰基苯酚、en=乙二胺)。通过超滤、高效液相色谱及非变性凝胶电泳对产物进行分离提纯,并用紫外-可见分光光谱、质谱及圆二色谱对其进行了表征。荧光性质研究表明,在室温条件下,不对称席夫碱配合物中电子发生了π*→π、π*→n跃迁,在490 nm处出现强的荧光发射峰;不对称席夫碱化合物Ln(n=2,3,4)对Cd2+均具有良好的选择性,最低检测浓度为3.6×10-5 mol/L;酸碱度研究表明,不对称席夫碱化合物Ln(n=2,3,4)探针在p H=9时对Cd2+检测具有最高灵敏度。更多还原

【Abstract】 According to the principle of Watson-Crick base pairing, a series of asymmetric Schiff base complex [Zn2Lnen Cl2] are synthesized step to step via DNA Template,(L1=D3-4M-D4-4M, L2=D3-4M-D4-4H, L3=D3-4M-D4-4T, L4=D3-4M-D4-4Cl, D3=C182H236O108N71P18、D4=C182H239O111N66P18, 4M=4-Methyl-2,6-diformylphenol, 4H=2-hydroxyisophthalaldehyde, 4T=4-tert-Butyl-2,6-diformylphenol, 4Cl=5-chloro-2-hydroxyisophthalaldehyde, en=ethanediamine). The products are purified and separated by centrifugation ultrafiltration, HPLC and gel-electrophoresis method, and characteried by UV- vis spectra, mass spectra and Circular dichroism Spectroscopy. The fluorescence studies at room temperature indicate that the asymmetric Schiff-base complexs emit strong fluorescence at about 490 nm, which can be probably assigned to π*- π or π*- n electron transitions. Furthermore, three different asymmetric Schiff-base compounds Ln(n=2, 3, 4) can act as probes, which have preferable recognition for Cd2+, with the minimum detectable density 3.6×10-5 mol/L. The p H- study shows the most sensitive detection p H value to Cd2 + is 9.更多还原