【作者】 张燕；

【导师】 代红军；

【作者基本信息】 宁夏大学 ， 蔬菜学， 2015， 硕士

【摘要】 本研究以6年生酿酒葡萄赤霞珠(Vitis vinifera L. Cabernet Sauvignon)和梅鹿辄(Vitis vinifera L. Merlot)为试材,系统研究了葡萄生长发育过程中白藜芦醇的变化规律。同时探讨了外源水杨酸、乙烯利处理对葡萄果实发育过程中白藜芦醇合成的诱导作用及与白藜芦醇合成相关的酶活性的关系。初步阐明了诱导子促进酿酒葡萄果实中白藜芦醇合成的积累机制,筛选出了提高酿酒葡萄果实成熟期白藜芦醇含量的最佳诱导子及其最适宜浓度和最佳使用时间,为从葡萄成熟浆果中获取高价值活性物质白藜芦醇提供理论依据。主要研究结果如下：1.在果实发育过程中,Res含量先增加后减少,L-苯丙氨酸(L-Phe)含量持续增加,苯丙氨酸裂解酶(PAL)、肉桂酸-4-羟基化酶(C4H)、4-香豆酸辅酶A：连接酶(4CL)、白藜芦醇合酶(RS)均只有一个活性高峰,且它们的变化存在着伴随性。L-Phe、PAL、C4H、4CL、RS活性变化与均Res含量变化相关系数均达到0.6以上,苯丙氨酸聚丙二酸盐代谢途径酶系活性及底物含量变化与Res含量变化具有较高的相关性,密切相关。2.100 mg·L-1外源水杨酸可以显著提高2个葡萄品种果实中Res含量,但是对不同时期诱导葡萄果实Res合成能力存在显著差异,其中成熟期诱导率最高,为最佳使用时间。对不同品种葡萄Res诱导合成能力也存在差异,诱导梅鹿辄Res合成的效果显著高于赤霞珠。3.对于两个供试品种,乙烯利诱导后,提前葡萄转色,并显著提高Res含量。梅鹿辄葡萄在成熟期诱导率较高,最适宜浓度为900 mg·L-1,赤霞珠转色期诱导率较高,最适宜浓度为600mg·L-1。4.50 mg·L-1水杨酸与300 mg·L-1乙烯利可明显促进果实中糖分、单宁、色素、酚类物质的积累,是提高梅鹿辄果实品质的最适宜浓度；100 mg·L-1水杨酸和600 mg·L-1乙烯利是提高赤霞珠果实品质的最适宜浓度。更多还原

【Abstract】 Taking wine grape of Vitis vinifera L. Cabernet Sauvignon of 6-year growth and Vitis vinifera L. Merlot as experimental materials, this research has systematically studied the transformation laws of resveratrol during growth of the grapes, and has discussed the induction functions of resveratrol synthesis and relations between resveratrol synthesis and its related enzymatic activity during growth of grapes by using exogenous salicylic acid and ethephon. This research has primarily explained that the inductor can enhance the accumulation of resveratrol synthesis in wine grape berries. The best inductor, most appropriate concentration and best timing of resveratrol content has been screened out to improve the fructescence of wine grapes, which has provided the theory basis of obtaining high value of active substance material-resveratrol from ripened wine grapes. The main study results are as follows:1. During the growth of berries, Res content has been increased at first and then increased, L-Phe content as increased continuously, PAL, C4H,4CL and RS has one active peak with the concomitant f their variations. The variation correlation coefficient of active variation of L-Phe, PAL, CH4,4CL, and RE and average Res content variation have reached over 0.6. The content variation of enzyme system active and substrate by Phenylalanine poly propylene acid salt metabolic pathways has high correlation with Res content variation.2.100 mg·L-1 exogenous salicylic acid can improve Res content of two grape variety synthesis of grape with the highest inductivity and the best use of time during maturation stage,. Res inducting synthesis are different in different grape varieties, for example, Res synthesis effectiveness of Vitis vinifera L. Merlot is obvious higher than Vitis vinifera L. Cabernet Sauvignon.3. For these two experimental materials using ethephon to induct, the grapes color has changed prior to its normal time and Res content has been improved obviously. Vitis vinifera L.Merlot’s high inductivity is in its maturation stage with the best concentration of 900 mg·L-1 while Vitis vinifera L. Cabernet Sauvignon is in its veraison with most appropriate concentration of 600 mg·L-1.4.50 mg·L-1 salicylic acid and 300 mg-L"1 ethephon are the most appropriate concentration of improve the berry quality of Vitis vinifera L.Merlot, which can obviously improve the accumulation of sugar, tannin, pigment and phenolic substance in the berries; while 100 mg-L"1 salicylic acid and 600 mg·L-1 ethephon are the most appropriate concentration of improve the berry quality of Vitis vinifera L. Cabernet Sauvignon.更多还原