【作者】 董青；

【导师】 张克勤；

【作者基本信息】 苏州大学 ， 纺织工程（专业学位）， 2015， 硕士

【摘要】 皮肤是人体的重要组成部分,它与我们的体温控制,人体免疫力以及人体与外界的传感等重要功能息息相关。创伤是我们不可忽视的一类疾病,伤口敷料一直是生活以及医药领域的必需品。传统敷料已不再满足人们的要求,所以近年来,可降解性合成聚合物纳米纤维敷料成为大家研究的重点。聚乳酸(PLA)有着难以比拟的优良生物降解性、生物相容性和一定的强度,广泛应用于生物医药领域。近来,有关石墨烯的抗菌性以及其细胞毒性的研究很多,结果表明石墨烯具有很好的抑菌效果且对细胞无毒性,同时氧化石墨烯有着很好的亲水性能,这为石墨烯在生物医药领域的应用提供了坚实的理论基础。目前为止,尚未发现有关将石墨烯加入聚乳酸进行静电纺丝,应用于生物伤口敷料的相关报道。本论文采用静电纺丝的方法制备出石墨烯改性聚乳酸(PLA)纳米纤维膜,探究石墨烯对纳米纤维膜性能的影响,探索其在生物伤口敷料应用潜力。首先,我们用层间催化解离法(Interlayer catalytic exfoliation,ICE)制备石墨烯,并对石墨烯进行SEM、TEM、FT-IR、Raman、XRD等测试。测试结果表明石墨烯具有较大的尺寸,而且厚度小于2nm。最终得到的石墨烯几乎没有缺陷,保留了其原有的完整基本晶面。接着,我们对纯的PLA进行静电纺丝工艺的探讨,得出如下结论:环境参数:温度为25℃,相对湿度为25%;纺丝液浓度;15%;过程工艺参数:电压为15kV喂料速率为0.5ml/h,针头到收集装置的水平距离为15cm;使用锡箔纸为最终收集装置。运用这一工艺参数纺制了石墨烯改性纳米纤维膜,对单根纤维进行了SEM以及TEM测试,结果表明石墨烯被包裹在纤维内部。对纳米纤维膜进行了FT-IR、Raman以及XPS测试,再次表明膜中含有石墨烯。最后,我们对复合膜的抗菌性能进行了测试。先对石墨烯及氧化石墨烯进行了抑菌率测试,结果发现石墨烯几乎不抗菌,而氧化石墨烯的抑菌率大于90%。然后分别对掺杂石墨烯与氧化石墨烯的静电纺丝膜进行抑菌圈实验,结果发现掺杂石墨烯的静电纺丝膜没有出现抑菌环,而掺杂氧化石墨烯的静电纺丝膜出现了一小圈的抑菌环,但是很小,可能是由于膜中氧化石墨烯含量太少导致的。为将其应用在敷料方面,需要对其进行更加深入的探索研究。更多还原

【Abstract】 Skin is vital component in human body. It has closely linked with body temperature,immunity, sensing outside and other important functions. Trauma is a kind of disease which cannot afford to ignore, so wound dressing is always indispensable in both living and field of medicine. However, traditional wound dressing no longer satisfies our needs,so degradable biologic dressing becomes research emphasis in recent years. Polylactic acid is widely applied in the field of biological medicine which has incomparable biodegradability, biocompatibility and certain strength. Recently, there are so many researches on antibacterial property of graphene and its cytotoxicity. The results show that graphene has great antibacterial effect and avirulence to cells. At the same time, grapheme oxide also owns excellent hydrophilic property, which all provides solid theoretical basis for application in the field of biological medicine. Up to now, we still have not found relevant report on making graphene into polylactic acid by electrospinning to apply to biological wound dressing.In this paper,we produce graphene modified polylactic acid nanofiber membrane by electrospinning.Then we explores the influence of graphene on membrane properties of nanofiber and its probable utilization on biological wound dressing.Firstly, we use Interlayer catalytic exfoliation(ICE)to produce graphene and do texts such as SEM,TEM,FTIR,Raman,XRD. The results show that graphene has larger size and its thickness is less than 2nm. Finally, we get flawless graphene which keeps its intact basic crystal planes.And then, we do technological discussion on electrospinning of polylactic acid andreach the following conclusion: environmental parameter: temperature is 25 ℃, relative humidity is 25%, and the concentration of spinningfluid is 15%. Processive technological parameter: voltage is 15 kV, feeding speed is 0.5ml/h, horizontal distance between needle to gathering unit is 15 cm. Use silver paper as final gathering unit. By applying this technological parameter to spin out graphene modified polylactic acid nanofibrous membrane, and doing SEM and TEM texts on single fiber, the results show that graphene is packed inside the fiber. And then we do FTIR and Raman texts on nanofiber membrane,the results show that membrane contains graphene again.Finally, we discuss the antibacterial properties of composite membrane.The results of antibacterial rate test show that graphene owns no antibacterial properties, while bacteriostasis rate of graphene oxide is over 90%. Then we do inhibition zone tests on nanofiber membrane,and the results show that composite membrane containing graphene has no bacteriostasis circle while composite membrane containing graphene oxide has a small circle of bacteriostasis circle. The circle is quite tiny, maybe because of little content of graphene oxide in the membrane.更多还原