【作者】 张峰；

【导师】 申才良；

【作者基本信息】 安徽医科大学 ， 外科学， 2015， 硕士

【摘要】 目的观察骨髓间充质干细胞的条件培养基(BMSC-CM)局部移植对脊髓损伤修复的作用。方法采用改良的Allen装置制作大鼠T9脊髓损伤模型。随机分为对照组(脊髓损伤后局部微量注射生理盐水10μl)和实验组(脊髓损伤后局部注射BMSC-CM 10μl),两组均采用BBB评分、斜板实验法观察大鼠运动功能恢复情况,苏木精-伊红(HE)染色观察脊髓损伤处血管生长及空洞体积的改变情况。结果BMSC-CM局部移植组的大鼠BBB后肢功能评分、斜板的倾斜角度明显高于对照组(P<0.05),脊髓损伤处的空洞体积明显小于对照组,血管直径大于对照组。结论在彻底避免了BMSCs的直接和间接作用下用BMSC-CM能够减小脊髓损伤处的空洞体积和促进血管生长,从而促进脊髓损伤后神经功能的恢复。更多还原

【Abstract】 Objective To investigate the promoting effects of bone marrow mesenchymal stem cells conditioned medium(BMSC-CM) local transplantation on function recovery of rat spinal cord injuries.Methods The spinal cord of adult rats was injured with modified Allen method, and the rats were randomly divided into 2 groups. The rats in the control group were subjected to the microinjection of saline solution(10 μl) into the SCI site after SCI, and those in the experimental group were injected with the concentrated conditioned medium(10 μl) derived from the passage 3 BMSCs. The inclined plane test and Basso,Beattie and Bresnahan locomotor rating scale(BBB scale) were used to observe the recovery of rats’ behavioral function after the transplantation. Hematoxylin-eosin(HE)staining to reflect the change of cavity volume and angiogenesis at the lesion epicenter.Results BMSC-CM treated animals show better BBB scores and inclined plane scores than control animals, and this difference was statistically significant(P<0.05).BMSC-CM treated group shows reduced cavity volume and the blood vessels exhibited larger diameters compared to control group.Conclusion BMSC-CM can promote the recovery of neurological function in spinal cord injury, reduce cavity volume, and improve blood vessel growth.更多还原