【作者】 张梅；

【导师】 董湘玉；

【作者基本信息】 兰州大学 ， 内科学， 2014， 硕士

【摘要】 目的：观察大鼠采用不同剂量阿奇霉素灌胃后,全胃半排空时间及血浆胃动素浓度的变化,揭示阿奇霉素致胃肠道症状的可能机制。方法：80只雄性wistar大鼠随机分为对照组(8只)和观察组(72只)；观察组按给药剂量分为小剂量、中等剂量、大剂量组,每组按给药时间分为1d组、3d组、6d组。观察组大鼠采用阿奇霉素灌胃,小剂量组、中等剂量组、大剂量组各组灌药剂量分别为3mg/100g、6mg/100g、12mg/100g。所有大鼠实验当天先用SPECT行胃排空检测,试餐为含有99mTc-DTPA的牛奶,用专用软件分析原始采集数据,得到全胃半排时间(GET1/2)、50分钟末残留率百分比、胃排空曲线。胃排空检测结束后,经腹主动脉采集动脉血2m1,运用放免法测定血浆胃动素浓度。用统计软件SPSS17.0进行统计分析,计数资料以“均数±标准差”表示,p<0.05为差异有显著性,p<0.01为差异有极显著性意义。结果：小剂量组、中等剂量组、大剂量组全胃半排时间明显缩短,与正常对照组(64.21±7.24)min比较,有统计学意义(p<0.05)。组内不同时间点比较无统计学意义(p>0.05)。观察组血浆胃动素浓度,小剂量组与正常对照组比较无统计学意义(p>0.05)；中剂量组、大剂量组与正常对照组比较明显增高,差异有统计学意义(p<0.05)。结论：阿奇霉素可促进胃排空。阿奇霉素促进胃排空的机制可能为小剂量下仅为胃动素样作用；中等剂量、大剂量时除胃动素样作用外,有内源性胃动素参与。更多还原

【Abstract】 OBJECTIVES:To investigate the effects of azithromycin on gastric emptying and plasma motilin in mouse, thus exploring mechanism of azithromycin to Gastrointestinal Symptom.METHODS:Eighty wistar rats were randomly dividing in10groups, i.e., the normal control group, small doses1-day group, small doses3-day group, small doses6-day group, normal doses-day group, normal doses3-day group, normal doses6-day group, large doses1-day group, large doses3-day group, large doses6-day group, and8rats in each group. The rats of observer groups were gastrogavaged by azithromycin. The dosages of small, normal and large groups were3mg/100g,6mg/100g and12mg/100g, respectively. First, gastric emptying was measured in unanesthetized rats by single photon emission computed tomography (SPECT) after gavaged milk loaded with technetium-99m diethylenetriamine pentaacetic acid(99mTc-DTPA), the gastric half-emptying time were calculated. Subsequently, blood was collected from the abdominal aorta to measure plasma motilin.RESULTS:In comparison to rats in control group,GET1/2decreased significantly in observer groups. The concentrations of plasma motilin in normal and large groups increased significantly, comparing with control group. However, there was no significant difference in small dose group.CONCLUTION:Azithromycin could promote gastric emptying. The mechanism of it not only are motilin-like effection, but also the participation of endogenous motilin.更多还原