【作者】 霍艳丽；

【导师】 黄雅钦；

【作者基本信息】 北京化工大学 ， 生物仿生材料， 2007， 硕士

【摘要】 目前,组织工程多孔支架的制备一直是组织工程研究的重点。本文对明胶基组织工程多孔支架的制备方法及其性能进行了系统的研究。首先,采用冷冻致孔真空干燥法及冷冻萃取常压干燥法制备了明胶基组织工程多孔支架,并对影响其结构与性能的因素(如明胶溶液浓度、交联剂用量、体系pH值等)进行了系统的考察。实验结果表明：两种方法制备的明胶组织基组织工程多孔支架都具有三维孔洞结构；所制备的支架平均孔径可达100pm以上,吸水率可达30倍；冷冻萃取常压干燥法与冷冻致孔真空干燥法相比,避免了耗时耗能的真空干燥过程,是一种节能环保的新方法。其次,对搅拌发泡-冷冻干燥法制备明胶基组织工程多孔支架进行了初探。实验结果表明：搅拌发泡-冷冻干燥法可以制备明胶基组织工程多孔支架；该支架中既具有利于细胞透过、生长与附着的相互连通的大孔,也具有利于营养物质的输送的小孔；将稀土铈引入支架所制备的稀土铈-明胶多孔支架对金黄色葡萄球菌的抑菌活性显著,且随着稀土铈浓度的增加,其抑菌活性逐渐增大。更多还原

【Abstract】 In recent years, preparation of porous scaffolds for tissue engineering is the key point of study in tissue engineering. In this paper preparation methods of porous gelatin based scaffolds for tissue engineering and their properties were systematically investigated.Firstly, techniques of freeze-drying in vacuum condition and freeze-extraction in atmospheric condition were used to prepare porous gelatin based scaffolds for tissue engineering and factors (such as gelatin solution concentration, volume of crosslinking agent, pH value of the system) which influence structures and properties of the scaffolds were investigated. Results showed that three-dimensional porous gelatin based scaffolds were successfully prepared by these two techniques; The average pore size could reach over100μm and water-absorb ratio could reach30times; Compared with freeze-drying technique, freeze-extraction was a time and energy saving technique because by this technique, time and energy consuming process of freeze-drying in vaccum condition can be saved.Then, stirring-foaming freeze-drying technique was tentatively used to prepare porous gelatin based scaffolds. Results showed that three-dimensional porous gelatin based scaffolds were successfully prepared by this technique. Structure of large pores benefit for cells penetration, propagation and adhesion and minor pores benefit for nutrients delivery was contained in the scaffold. It was found that the bacteriostatic activity of cerium nitrate-gelatin scaffolds against Staphylococcus aureus was outstanding, and increased with the increase in the cerium nitrate concentration.更多还原