【作者】 史洪涛；

【导师】 边传周；

【作者基本信息】 河南农业大学 ， 兽医， 2014， 硕士

【摘要】 当前畜禽业大量使用抗生素所带来的危害表现得越来越突出，研发无残留、无污染的绿色饲料添加剂代替抗生素成为畜牧业研究的热点。本研究首先用黄芪水提物与营养肉汤培养基按比例进行组合，获得最佳培养基配方和最适培养条件；再将发酵制剂以不同的剂量添加到肉鸡的饮水中，研究其在肉鸡生产上的应用效果；并对发酵制剂中枯草芽孢杆菌的稳定性和发酵制剂的安全性进行测定。主要结果如下：黄芪水提物发酵培养枯草芽孢杆菌的最适培养基为：每升液体培养基中含黄芪水提物60%，营养肉汤40%，硫酸铵0.4g，氢氧化钠调节pH值；最适培养条件为：37℃，初始pH值8.0，装液量100mL（500mL三角瓶），接种量3%，恒温摇床转速160r/min。黄芪-枯草芽孢杆菌发酵制剂对肉鸡生产性能、免疫器官指数、免疫效果影响结果:发酵制剂能提高肉鸡平均日增重和日采食量，降低料肉比；与对照组相比，适宜的添加剂量可以提高21、42日龄肉鸡的脾脏指数、法氏囊指数、ND和AI抗体滴度。结果表明，黄芪-枯草芽孢杆菌制剂能提高肉鸡的生产性能和免疫功能，完全可以替代抗生素使用。贮存时间对发酵制剂活菌稳定性和安全性的影响结果：保存不同时间的发酵制剂中活菌数目测定结果表明，保存5个月后枯草芽孢杆菌存活率在95%左右，稳定性较高；不同日龄肉鸡大剂量饮水给药结果表明，发酵制剂安全性高。更多还原

【Abstract】 Nowadays, it is a hot spot that research and development of the greenfeeding additives, which has always been to replace the role of antibiotics, withoutresidue and pollution in animal husbandry, with the more and more prominent dangerby the mass use of antibiotics. In this study, the optimum medium formulation andculture condition were obtained, firstly, by different proportions of watery extract ofAstragalus root in Nutrient-Broth-Medium. Then, different doses of the fermentedpreparation was added in the water of broilers, to research the effect of that inproduction. At last, the stability of Bacillus subtitles in the fermented preparation wasassayed, as well as the security of fermented preparation. The main research content isas follows：Bacillus subtitles was cultured with watery extract of Astragalus root, and theoptimum medium formulation and culture condition were identified. Results showedthat optimum fermentation medium component for Bacillus subtilis was wateryextract of Astragalus root60%, Nutrient-Broth-Medium40%, ammonium sulfate0.4g.The obtained best fermentation condition was described as: temperature37℃,original pH8.0, amount of inoculation3%, amount of load100ml(in500ml,flask),rotation speed160rpm．To observe the effect of the fermented preparation of Astragalus-Bacillus subtilis,on productivity, immune organ index and immune effect of vaccine on broilers.Theresults as follows:The fermented preparation can improve the broilers’ daily weightgain and the average daily feed intake.What’s more, the feed conversion rate wasreduced.Compared with control group, the fermented preparation on suitable dosagecan improve the spleen index,the bursal index,the ND and AI antibody titer of the broilers on21and42days of age.The results indicated that the fermented preparationof Astragalus-Bacillus subtilis can be an ideal alternative to antibiotics, as enhancingthe productivity and immunological function of broilers.To study the influence of storage time on the stability and safety of viablebacteria in fermented preparation, we determined the amount of viable bacteria fromdifferent fermented preparation saved on different days. At the same time, High dosefermented preparation was drank by broiler with different ages. The results suggestedthat the survival rate of Bacillus subtitles was around95%after conserved5months,with high safety.更多还原