【作者】 王莉；

【导师】 韩渊怀；

【作者基本信息】 山西农业大学 ， 植物学， 2014， 硕士

【摘要】 突变体是功能基因组学研究的重要工具之一。鉴于谷子全基因组测序已经完成,而对其基因功能的探索急需丰富的突变体群体,本论文利用不同剂量(4Gy、8Gy、16Gy和32Gy)的快中子对豫谷一号进行辐射处理,从M1代收获谷穗保存,4个剂量各收获株系6000、7000、6000和5000株,构成突变体库基础资源。M2代按剂量分别播种,并从苗期到成熟期观察突变,发现的表型包括：白化、黄化、小穗、高秆、矮秆等。对这些突变体进行单株收获,以便后期从遗传和分子水平上进行深入研究。针对本项目发现的矮化突变体以及矮化在谷子生产和育种中的重要性,本文对谷子中四个与水稻SD1基因同源的矮化基因从基因结构,上游3000bp启动子区域作用元件进行了分析,发现这些基因的作用元件有很大的差异,从而可能调控其不同的表达水平和模式；并将这些基因与水稻、高粱、玉米、柳枝稷和二穗短柄草共六种禾本科植物的同源基因进行比对,构建遗传进化树图谱。谷子快中子突变体库的建立将有助于谷子功能基因组学的研究,为发掘与利用谷子基因资源奠定了材料基础。更多还原

【Abstract】 Mutants are important resources for functional genomics. Since the full genome of foxtail millet was sequenced and there is an urgent need for mutant populations for gene function study, this study applied fast neutron treatment on foxtail millet variety Yugul at four doses:4Gy,8Gy,16Gy and32Gy, single panicles were harvested, a total of6000、7000、6000and5000lines were obtained for each dose, respectively, establishing the basic resources of the mutant library. M2generation was grown for each dose and the corresponding populations were screened for visible phenotype from seedlings to mature stage. Mutants showing albino, yellow leaves, changed spikelets, higher and dwarf and so on. These mutants were harvested by single panicles for further genetic and molecular analysis. Based on the fact that dwarf mutants were identified in this study and the importance of dwarf resources in the production and breeding of foxtail millet, this thesis analysed the four homologue genes in foxtail millet to the rice dwarf gene SD1, by investigating their gene structures and cis elements in their promoter region (3000bp upstream of transcription start site). It was found that there were strong difference in the number and types of cis elements among the promoter regions of the four genes, indicating potentially different expression levels and patterns of these four genes. The phylogenetic relationship of these four SD1homologues among graminaceous was analysed and phylogenetic tree constructed.The construction of fast neutron mutant library would promote the research on functional genomics of foxtail millet and lay the foundation for exploring and utilizing the genetic resources of the crop.更多还原