【作者】 李莉；

【导师】 高建；

【作者基本信息】 重庆医科大学 ， 内科学， 2014， 硕士

【摘要】 目的:分析micro-RNA(miR-499和miR-149)单核苷酸多态性及其表达水平与消化道肿瘤易感性的关系。方法:在PubMed、 Wiley Online Library、Web of Science、中国知网等数据库上检索关于micro-RNA的表达与消化道肿瘤关系的病例-对照试验的文献，采用Review Manager Version5.1及STATA/SE软件12.0进行Meta分析。结果:总共检索到关于miR-499的文章11篇，关于miR-149的文章6篇。对于miR-499多态性，纯合子可增加人群患结直肠癌的风险性(GGvs. AA: OR=1.66,95%CI:1.02–2.70, Ph=0.10, P=0.04)。在亚组分析，未发现miR-499与不同人种患消化道肿瘤的有所关联，且miR-499与患肝癌、胃癌的风险性无关。对于miR-149多态性，隐性遗传模型可能增加消化道肿瘤的风险性(TT vs. TC+CC: OR=1.15,95%CI:1.03–1.30,Ph=0.68, P=0.02)。在亚组分析，miR-149多态性可能增加亚洲人口患消化道肿瘤的风险性(TT vs. TC+CC: OR=1.14,95%CI:1.01–1.29, Ph=0.79, P=0.03)。结论:MiR-499单核苷酸多态性可能增加人群患结直肠癌的风险性，miR-149单核苷酸多态性可能增加人群患消化道肿瘤的风险性，特别是亚洲人口。更多还原

【Abstract】 Objective：To investigate the association between two miRNApolymorphisms (miR-499and miR-149) and gastrointestinal (GI) cancerrisk.Methods：We conducted a search of case-control studies in PubMed,Wiley Online Library,Web of Science and the CNKI database. We usedCochrane Review Manager Version5.1and Stata/SE software12.0toanalyze the data from each study.Results：Eleven rs3746444studies and six rs2292832studies wereincluded in our meta-analysis. The only obvious association between themiR-499polymorphism and colorectal cancer susceptibility was found inthe homozygote comparison (GG vs. AA: OR=1.66,95%CI:1.02–2.70,Ph=0.10, P=0.04). No signifcant association was found in the subgroupanalysis for ethnicity and risk of hepatocellular and gastric cancer. Amarginally elevated GI cancer risk was discovered in the recessive modelfor miR-149(TT vs. TC+CC: OR=1.15,95%CI:1.03–1.30, Ph=0.68, P =0.02). Stratifying the results by ethnicity revealed a slight associationbetween the recessive model and the Asian population (TT vs. TC+CC: OR=1.14,95%CI:1.01–1.29, Ph=0.79, P=0.03).Conclusion：The present meta-analysis indicates that miR-499may beassociated with the risk to colorectal cancer. MiR-149may confer amarginally increased risk of susceptibility to gastrointestinal cancer,especially for Asians.更多还原