【作者】 杨燕；

【导师】 饶高雄； 左爱学； 孙赟；

【作者基本信息】 云南中医学院 ， 中药学， 2014， 硕士

【摘要】 本论文由六章组成。前三章主要论述了云南产栽培云南红豆杉（Taxus yunna-nensis Cheng et L.K.Fu）枝叶、黄龙尾（Agrimonia pilosa Ledeb. var. nepalensis (D. Don)Nakai.）、芜菁还阳参（Crepis napifera (Franch.) Babc.）的化学成分研究；后三章介绍了云南省红豆杉资源及云南红豆杉研究概况，并对龙芽草属及还阳参属药用植物资源、化学成分、生物活性、临床应用等方面进行了较全面的综述。运用吸附树脂、正相硅胶、反相硅胶、凝胶柱层析等分离方法，结合波谱技术，从以上三种药用植物中共分离鉴定62个化合物，化合物结构类型包括紫杉烷二萜、三萜、黄酮、倍半萜、木脂素等类型，其中有2个新化合物。云南省是云南红豆杉资源主要分布区，拥有得天独厚的物种资源和环境优势。但目前云南省相关产业只处于原料生产提供的阶段，从栽培云南红豆杉（T.yunnanensis）枝叶的乙醇提取物中共分离得到30个单体化合物，主要结构类型包括紫杉烷二萜、黄酮、甾体。蔷薇科龙芽草属植物黄龙尾（A. pilosa Ledeb. var. nepalensis）是云南省习用的道地药材，具有收敛止血、调经止带的功效。为提高“黄龙尾”内在物质基础的认识，进一步完善提高“黄龙尾”药材质量标准，首次对其进行化学成分研究。从黄龙尾全草的乙醇提取物中分离得到23个单体。结构类型包括三萜、黄酮、间苯三酚衍生物、异香豆素、植物甾醇类和脂肪酸，所有化合物均是从该植物中首次分离得到。药材“芜菁还阳参”在各版《云南省中药材标准》中只有对照药材的TLC鉴别，没有明确成分的薄层鉴别和含量测定项。为加强对于芜菁还阳参物质基础的认识，以利于进一步开展“芜菁还阳参”药材质量研究，对“芜菁还阳参”药材进行化学成分研究。从芜菁还阳参（C. napifera）干燥根中分离得到9个单体，主要结构类型包括三萜、倍半萜及木脂素等，其中包括1个新的愈创木烷型倍半萜内酯苷及1个新的苯甲酸类化合物：芜菁还阳参苷、3,5-二羟基-4-甲氧基-苯甲酸-2-C-葡萄糖苷（3,5-Dihydroxy-4-methoxy-benzoic acid-2-C-glucoside）。论文后三章介绍了目前云南省红豆杉资源及云南红豆杉的研究概况，并对龙芽草属及还阳参属药用植物资源、化学成分、生物活性、临床应用等方面进行了较全面的整理总结。更多还原

【Abstract】 This master’s thesis is composed of six chapters. The chapters1-3express the studieson chemical constituents of Taxus. yunnanensis Cheng et L.K.Fu., Agrimonia pilosaLedeb. var. nepalensis (D. Don) Nakai., Agrimonia pilosa Ledeb. var. nepalensis(D.Don)Nakai; the chappters of4-6, give a summary of the resources, chemical constituents,bioactivities, clinical application of Taxus. Yunnanensis, Agrimonia, Crepis.Sixty two compounds, including2new compounds were isolated from the abovethree species of plants by various chromatographic methods: silica gel, Rp-18, sephadexLH-20, et al. These compounds are composed of taxoids, triterpenoids, flavonoids,sesquiterpene, lignans, et al.Yunnan Province, in which Taxus resources are mainly distributed, is rich in naturalresource and has the diversity of plant life. But the relative industry of Taxus in YunnanProvince just provide the raw medicine. In order to promote development of taxanes,research on the branches and leaves of Taxus yunnanensis.was carried out. And38compounds were isolated from the ethanol extract of Taxus yunnanensis. The compoundsreferred taxane, diterpenoid, flavonoids, steroids, and a new phloroglucinol.Agrimonia pilosa Ledeb. var. nepalensis was usually used for the treatment ofbleeding, gynecological diseases, and gastrointestinal diseases in Yunnan Province. Inorder to understand the effective ingredients and prefect the quality standards, the researchon chemical constituents of whole plant was carried out for the first time. And23compounds were isolated from the extract, including triterpenoids, flavonoids,phloroglucinol derivatives, isocoumarins, phytosterols, and fatty acids.Radix Crepidis Napiferae was recorded in each version of Traditional ChineseMedicine Standard in Yunnan Province,There is only the TLC method ofstandard herb,and no compound identification test and content assay. So, we research on the radix ofCrepis napifera to improve quality standards of Radix Crepis napifera. And9compoundswere isolated from the ethanol extract, including triterpenoids, sesquiterpenes and lignans.Besides, two new compounds were isolated.In chappters4-6, reviews on the resource, chemistry, bioactivity, and clinical application of genus Taxus.genus, Agrimonia, and genus Crepis were presented.更多还原