节点文献
规模猪场猪瘟与猪蓝耳病免疫效果评估及评估模型建立
The Immune Effect Assessment of Swine Fever and PRRS in Scale Pig Farms and Assessment Model Is Established
【作者】 王慧；
【导师】 傅胜才；
【作者基本信息】 湖南农业大学 ， 临床兽医学， 2013， 硕士
【摘要】 为了提高试验猪场免疫水平,降低猪瘟、猪高致病性蓝耳病(论文题目为蓝耳病系其简称)发病率,同时提出猪瘟、猪高致病性蓝耳病抗体水平预警参考指标,并构建了一套系统的免疫效果评估程序,供养猪生产者和基层动物疫病控制机构参考使用。选择5家规模猪场,调查其生产情况,并通过ELISA检测技术进行猪瘟、猪蓝耳病的免疫效果评估,调整不合理的免疫程序,30-40天后再进行抗体水平检测分析,以验证免疫程序调整是否合理,同时,提出猪瘟、猪高致病性蓝耳病抗体水平预警评价指标并建立一套动物疫病免疫评估模型,最后将此模型运用到10家小规模猪场。研究结果表明：(1)通过调查,了解到猪场存在免疫剂量和次数不合理、免疫程序生搬硬套、免疫工作被动盲目等现象。(2)5家规模猪场抗体水平评估结果发现均不合格。(3)免疫程序调整后,5家规模猪场的抗体水平都得到了显著提升,猪群健康状况明显改善。(4)提出了猪瘟、猪高致病性蓝耳病抗体水平预警评价指标并总结出了一套简便易行的免疫评估模型。(5)通过将此评估模型应用到10家小规模猪场,均得到了良好的验证,值得养猪生产者推广应用。
【Abstract】 In order to improve the immune effect and reduce the incidence of CSF and HPRRS(Paper topics for PRRS is due to its abbreviation),At the same time,Set up the warming reference of CSF and HP-PRRS antibody Ievel,And to establish a systematic to evaluate immune effect by using this model can make the immune effect assessment an orderly way.This research select five scale pig farms to investigate production situation and to through the ELISA test to analysis the antibody level, Then adjusted the unreasonable of immunization programs and analyse the antibody level again after30to40days, to verify that the immune adjustment is or not reasonable. Meanwhile set up the warming reference of CSF and HP-PRRS and the immune evalution model. The last put the model into ten small scale pig farms to use to.The result indicates that (1)through the survey of five scale pig farms, Know that phenomenon of immunization time and the immune dosage are unreasonable, Blind imitation and passive;(2)We found that five scale pig farms for the detecting antibody levels were unqualified, A and B in the HP-PRRSV poisonous infection stage, The antibody of HP-PRRS of C,D and E are not qualified. the A’s CSF level in a poor game and four ohers are relatively stable;(3)After adjust the immunization programs, that the antibody level all got corresponding improvement and improved pigs health.(4)Set up the warming reference of CSF and HP-PRRS and the immune evaluation model.(5)Through the evalution model is applied to10small scale pig farms, All achieved good results, That worthy of producers for application.