【作者】 李瑞；

【导师】 程玉芬；

【作者基本信息】 南昌大学 ， 妇产科学， 2013， 硕士

【摘要】 目的：比较不同子宫内膜中骨桥蛋白（Osteopontin，OPN）和整合素αv、β3的表达。探讨其在子宫内膜异位症（endometriosis，EM）不孕和发病机制中可能起到的作用。方法：利用免疫组化半定量检测内异症不孕患者和输卵管性不孕患者子宫内膜中OPN和整合素αv、β3的表达。结果：1.OPN在输卵管性不孕患者、内异症性不孕患者在位内膜中的表达分泌期均高于增殖期，差异有显著性（P<0.05）。OPN在内异症不孕患者异位内膜中的表达增殖期和分泌期无显著性差异（P>0.05）。2.分泌期OPN的表达：输卵管性不孕患者高于内异症性不孕患者在位内膜，差异有统计学意义（P<0.05）。分泌期整合素αv的表达：输卵管性不孕患者子宫内膜高于内异症性不孕患者在位内膜，但差异没有统计学意义（P>0.05）。3.内异症性不孕患者异位内膜OPN的表达高于其在位内膜，差异有显著性（P<0.01），输卵管性不孕患者子宫内膜OPN的表达高于内异症性不孕患者在位内膜OPN的表达，差异有显著性（P<0.05）。内异症性不孕患者异位内膜整合素αv的表达高于其在位内膜整合素αv的表达，差异有显著性（P<0.01），内异症不孕患者异位内膜整合素αv的表达高于输卵管性不孕患者子宫内膜整合素αv的表达，差异有显著性（P<0.05）。输卵管性不孕患者的子宫内膜和内异症性不孕患者在位内膜均未见表达整合素β3，内异症性不孕患者异位内膜持续表达整合素β3。结论：OPN、整合素αv在内异症性不孕患者在位内膜分泌期的表达异常，可能是内异症不孕的原因之一。OPN、整合素αv、β3在内异症患者异位内膜中的异常表达可能是异位内膜可以成功种植的原因之一。更多还原

【Abstract】 Objectives:To study the expression of osteopontin and integrin αv, β3in endometria,and toinvestigate their role in endometriosis-associated infertility and pathogenesis ofendometriosis.Methods:The expression of osteopontin and integrin αv, β3in endometrium of patientswith tubal infertility and endometriosis infertility were determined by employingimmunohistochemical method.Results:The expression of osteopontin in patients with tubal infertility,endometriosisinfertility is that secretory phase were higher than the proliferative phase in eutopicendometrium, the difference was statistically significant(P<0.05).There were nosignificant differences between the expression of osteopontin in secretory phase andin proliferative phase in ectopic endometrium of patients with endometriosisinfertility(P﹥0.05).The expression of osteopontin in secretory phase is that the patients with tubalinfertility were higher than the patients with endometriosis infertility, the differencewas statistically significant(P<0.05).The expression of integrin αv in secretory phaseis that the patients with tubal infertility were higher than the patients withendometriosis infertility, but the difference had statistical significance(P>0.05).The expression of osteopontin in patients with endometriosis infertility is thatthe ectopic endometrium were higher than the eutopic endometrium,the differencewas statistically significant(P<0.01).The expression of osteopontin in patients withtubal infertility was higher than the patients with endometriosis infertility,thedifference was statistically significant(P<0.05).The expression of integrin αv inpatients with endometriosis infertility is that the ectopic endometrium were higherthan the eutopic endometrium,the difference was statistically significant(P<0.01).Theexpression of integrin αv in the ectopic endometrium of patients with endometriosis infertility were higher than the endometria of patients with tubal infertility,thedifference was statistically significant(P<0.05).The endometria of patients with tubalinfertility and the eutopic endometrium of patients with endometriosis infertilityshowed no expression of integrin β3.Both the proliferative phase and secretoryexpress integrin β3in ectopic endometrial of patients with endometriosis infertility.Conclusions:The abnormal expression of osteopontin and integrin αv in the secretory phase ofpatients with endometriosis infertility may be one of the causes of infertility inendometriosis. The abnormal expression of osteopontin, integrin αv, β3in the ectopicendometrium of patients with endometriosis may be one of the reasons that theectopic endometrium can be successfully grown.更多还原