【作者】 胡昕；

【导师】 蔡丽萍；

【作者基本信息】 南昌大学 ， 妇产科学， 2013， 硕士

【摘要】 目的:研究柚皮苷(Naringin)对人卵巢癌SKOV3细胞P38丝裂原活化蛋白激酶(P38mitogen activated protein kinases,P38MAPK)蛋白与细胞外信号调节激酶1/2(extra cellular signalregulated protein kinase,ERKl/2)蛋白表达的影响，初步探讨柚皮苷的抗肿瘤作用。方法:体外培养人卵巢癌SKOV3细胞,分为空白对照组、柚皮苷10μmol/L组、20μmol/L组、40μmol/L组、塞来昔布80μmol/L组。采用MTT比色法在48h测定不同浓度的柚皮苷对SKOV3细胞的抑制率，运用WesternBlot法测定培养48h后各组细胞中P38MAPK及ERK1/2蛋白表达水平。结果:1.MTT检测法显示，柚皮苷对卵巢癌SKOV3细胞生长具有明显的抑制作用。2.WesternBlot法结果显示，与空白对照组相比，低浓度柚皮苷组（10μmol/L、20μmol/L）对SKOV3细胞P38MAPK蛋白表达的无明显影响，而40μmol/L柚皮苷及塞来昔布组可明显下调P38MAPK蛋白表达,具有统计学意义（P<0.05）。3.WesternBlot法结果显示，与空白对照组相比，低浓度柚皮苷组（10μmol/L、20μmol/L）对SKOV3细胞ERK1/2蛋白表达的无明显影响，而40μmol/L柚皮苷及塞来昔布组可明显下调ERK1/2蛋白表达,具有统计学意义（P<0.05）。结论:柚皮苷能抑制人卵巢癌SKOV3细胞P38MAPK及ERK1/2蛋白的作用,提出适当浓度的柚皮苷能阻断人卵巢癌SKOV3细胞的生长，为今后临床治疗卵巢癌提供理论依据。更多还原

【Abstract】 Objective：To investigate effects of Naringin on expression of P38MAPK and ERK1/2signaling proteins of Human ovarian cancer cell line SKOV3,a preliminary to explorethe antitumor effect of naringin.Methods:SKOV3cell was cultured in vitro and divided into five groups randomly,namelycontrol group,naringin group(10μmol/L),naringin group(20μmol/L),naringin group(40μmol/L)and celecoxib group(80μmol/L).Growth inhibition rates of differentrosiglitazone concentrations for24h to SKOV3cells were measured by MTT method.The expression of P38MAPK and ERK1/2signaling proteins was determined byWestern blot after Forty-eight hours.Results:1.Naringin can significantly inhibit the growth of SKOV3cell.2.Compared with control group,only naringin group(40μmol/L)and celecoxibgroup can significantly cut P38MAPK signaling proteins,the difference wasstatistically significant(P<0.05).3.Compared with control group,only naringin group(40μmol/L)and celecoxibgroup can significantly cut ERK1/2signaling proteins,the difference was statisticallysignificant(P <0.05).Conclusions:Naringin can inhibit P38MAPK and ERK1/2signaling proteins of SKOV3cell,suggest appropriate concentration of naringin can prevent the growth of SKOV3cells,provide a theoretical basis for the clinical treatment of ovarian cancer.更多还原