【作者】 刘凯；

【导师】 刘波；

【作者基本信息】 河南大学 ， 生物物理， 2013， 硕士

【摘要】 壳聚糖是一种天然高分子的可再生资源，可以从虾、蟹、贝类等海鲜中获得。壳聚糖已被发现并得到广泛的应用，它具有许多独特的生物学特性：生物相容性，可生物降解为无害的产品，无毒，对蛋白质有着显着的亲和性，抗菌，止血，抑制真菌和抗肿瘤等。而分子自组装在生物工程和纳米科技领域有着广泛的应用。在最近的几十年里，分子自组装作为一种新的技术手段，在高新技术领域产生了重大的影响。本文探讨了壳聚糖分子自组装的基本原理、影响因素和研究进展，并对壳聚糖的分子自组装薄膜进行了深入的研究。作为一种具有生物相容及生物可降解性能的天然高分子化合物，人们对壳聚糖自组装薄膜在生物材料领域的认识日益深刻，对产品应用的开发也日益增强。最近的研究表明，壳聚糖自组装薄膜极有可能作为辅助材料，而被应用于组织工程学。这是由于壳聚糖自组装薄膜，具有多种组织工程学材料所必备的性能：如多孔结构，凝胶成型性能，易于化学改性，高亲和力体内大分子等。经过调查发现，人们更专注于壳聚糖自组装薄膜在生物医学方面的组织工程学应用，即皮肤，骨骼，软骨，肝脏，神经和血管的各类。而伤口愈合领域也一直是基于壳聚糖自组装薄膜医疗应用研究的一个主要重点。壳聚糖自组装薄膜所具有的特殊性能，有利于促进皮肤快速再生，加速伤口的愈合，其作用包括从简单的伤口修复到复杂的人造皮肤。在本文中，首先我们用新的方法制备出壳聚糖单分子层薄膜，并利用AFM对其做了表征。其次，我们选择了三种不同分子量的壳聚糖，对它们的分子自组装行为进行了深入的研究。通过利用AFM技术，在固定的培养时间下，研究了三种经过酸催化后的壳聚糖分子的自组装形态。AFM成像结果表明，分子量不仅会直接影响到壳聚糖分子的自组装路径，同时还会影响到三种分子量的壳聚糖终态薄膜的成膜类型。最后，我们制备了壳聚糖的复合纳米薄膜材料，并对其做了性能研究。本文通过对壳聚糖自组装薄膜与生物材料相关特性的分析，根据目前的研究成果，指出壳聚糖自组装薄膜是一种很有潜力的生物材料。在实验中，主要完成工作如下：1、利用AFM扫描技术通过调整壳聚糖溶液的浓度和培养时间，制备出超薄的单分子层壳聚糖生物薄膜。2、使用分子自组装方法制备得到壳聚糖的分子自组装薄膜。并通过利用AFM观察发现，壳聚糖分子量的大小会对自组装薄膜的特性产生影响。3、在前面研究的基础上我们还制备了壳聚糖的复合薄膜材料，并对其形成机理做了探究。更多还原

【Abstract】 Chitosan is a natural polymer from renewable resources, obtained from shell of shellfish, and thewastes of the seafood industry. Chitosan has been found a fascinating candidate in a broad spectrum ofapplications along with unique biological properties including biocompatibility, biodegradability toharmless products, nontoxicity, physiological inertness, remarkable affinity to proteins, antibacterial,haemostatic, fungistatic and antitumoral properties. Molecule self-assembly technology has been widelyapplied in biotechnology and nanotechnology. As a fabrication tool, molecular self-assembly technologywill become tremendously important in the coming decades. In this article, mechanism, influence factors,some research advances and application of chitosan film molecule self-assembly technology are studied.Chitosan is a natural polymer with biocompatibility and biodegradability. Recognition of the potentialutility of chitosan self-assembly film is growing and the field of chitosan biomaterials is poised toexperience rapid growth. Recent studies suggested that chitosan self-assembly film is promising candidatesas a supporting material for tissue engineering applications owing to their porous structure, gel formingproperties, ease of chemical modification, high affinity to in vivo macromolecules, and so on. In this review,people focus on the various types of chitosan self-assembly film and their use in various tissue engineeringapplications namely, skin, bone, cartilage, liver, nerve and blood vessel. The field of wound healing hasbeen a major emphasis in chitosan-based medical applications research. Chitosan self-assembly filmpossesses the properties favorable for promoting rapid dermal regeneration and accelerate wound healingsuitable for applications extending from simple wound coverings to sophisticated artificial skin matrices.In this paper, first we use new methods to preparation of chitosan monolayer film and by using theAFM characterization developed it. Secondly，three kinds of chitosan with different molecular weight werechosen to systematically investigate their self-assembly behavior. Through high resolution atomic forcemicroscopy (AFM) technique, we have characterized the self-assembled morphologies of three acidulatedchitosan molecules at designed culture time. The AFM characterization results suggest that the molecularweight has direct influence on the self-assembled pathway of chitosan molecules, therefore leading toformation of chitosan films with different properties. At last, we use different methods of preparation of chitosan composite film. And we explored the properties. This paper analyses the properties of chitosanself-assembly film which are important to biomaterials.In our experiments, the contents are listed as follows:1. Using AFM scanning technology by adjusting the concentration of chitosan solution and theincubation time, preparation of ultrathin monolayer chitosan biological membrane.2. The preparation chitosan film by molecular self-assembly method. Influence of molecular weighton the self-assembly film of chitosan studied by atomic force microscope.3. The preparation of composite films with chitosan, and the exploration of the mechanism.更多还原