【作者】 王宇；

【导师】 管荣展；

【作者基本信息】 南京农业大学 ， 作物遗传育种， 2012， 硕士

【摘要】 甘蓝型油菜是我国重要的油料作物,改变其品质和倒伏性是增加油菜产量的关键。本研究利用EMS诱变甘蓝型油菜NJ7982,筛选获得矮秆突变材料M176,它与高秆甘蓝型油菜杂交,获得了2个F2群体,对M176的株高数量性状进行遗传分离分析。还以M176和矮秆亲本J194的F2群体中的117个株系为材料,利用数量性状的思想,用公开发表和新开发的SSR标记对其株高性状进行QTL初步定位。该研究为油菜育种提供了降低植株高度和抗倒伏的有价值的新品种。主要研究结果如下：(1)遗传规律分析矮秆突变材料M176与高秆甘蓝型油菜杂交,获得了2个F2群体,对群体的不同株高进行IECM数量性状的遗传规律,遗传分离分析表明,株高性状受一对主基因控制,株高性状呈现部分显性,纯合矮秆的期望均值为65cm左右,杂合矮秆的平均株高77-92cm,显示矮秆基因具有明显降低株高的效应。得出结论,矮秆群体是由一对不完全显性的基因控制的。(2)引物多态性情况用759对已发表的SSR引物对两亲本M176(m1025矮秆亲本)和J194((高秆亲本)进行多态性筛选,有89对引物在亲本之间检测到多态性,占总标记数的11.7%。根据BAC第九染色体的相应位置设计新的SSR引物,共设计113对SSR引物,其中有8对在10株F2代中表现多态,但通过群体验证,稳定,清晰可读的多态只有3个其中BrA09Y21与矮秆基因连锁。(3)株高性状QTL定位用上述亲本间存在多态性的89对SSR引物对F2群体117个单株进行了基因型分析,发现一个新型矮秆基因,位于第九染色体,筛选得到5个和其连锁的标记,其中距离Na10G06为7cm,距离BrA09Y21为6.5cm。更多还原

【Abstract】 Brassica napus is ne of the most important oil crops in our country.Now,the improvement of plant form and loding resistanece is the key to increase the yield.The M176mutant with dwarfing plant height were raised by treatment of EMS inducement of canola line NJ7982(Brassica napus L).Dwarf mutant M176was crossed to lines with normal plant height,leading to its two F2populations. The two populations have a genetic segregation analysisThis research based on the F2population with117lines derived from the M176(Dwarf parents) and J194(normal tall parents) to locate the QTL gene. This research has provided an new valuable material for reduction of plant height and breeding for lodging resistance in Brassica napus L.The main results are as follows:1) Dwarf mutant M176was crossed to lines with normal plant height,leading to its two F2populations. The inheritance of the dwarf trait of M176was studied through IECM. Genetic segregation analysis showed that the trait of plant height (Brassica napus L) was controlled by one pair of major genes,the dwarfing trait showed the partial dominance to the higher,the expected average value of plant height for the homologous dwarf genotype was about65cm,and for the heterozygous genotype was77-92cm,indicating that the dwarf gene may significantly reduce the plant height.2) Previously Published and newly developed SSR markers using117F2lines derived from M176and J194,89of759Published SSR markers showed11.7%polymorphism between two parents’M176’and’J194’,113new primers was designed according to the Ninth chromosome of BAC,only3polymorphic band was found3) Totally759SSR primers were screened,five polymorphic band was found linked to the dwarf gene Bn176and mapped on linkage group N9.finally,the dwarf gene Bnl76was located between Na10G06and BrA09Y21.更多还原