【作者】 杨志；

【导师】 刘绍琴；

【作者基本信息】 哈尔滨工业大学 ， 生物医学工程， 2012， 硕士

【摘要】 作为构成碳的同素异形体石墨、碳纳米管及富勒烯的基本结构单元,石墨烯以其出色的电学、光学、热学、力学性能吸引了科学与工程各领域学者的广泛兴趣。然而,如何在实际应用中保留微观尺寸依赖性属性是现阶段面临的重要研究之一。本论文以石墨烯为原料,通过石墨烯溶胶与聚烯丙基胺盐酸盐(PAH)的逐层交替自组装成功构建了(PEI/PSS)2(PAH/PSS-G)100纳米复合膜材料,同时采用微纳加工原理完成薄膜图案化设计与加工。最后通过纳米复合膜材料与HepG2细胞的共培养考察薄膜生物相容性。本论文主要工作包括：(1)利用Hummer方法成功制备出以PSS为稳定剂的石墨烯溶胶,利用层层自组装技术成功实现了PAH稳定的石墨烯(PSS-G),聚烯丙基胺盐酸盐(PAH)的交替自组装,并在ITO导电玻璃、载玻片、石英、单晶硅等不同基体上构建了(PEI/PSS)2(PAH/PSS-G)100自支撑多层纳米复合薄膜。薄膜厚度均一、表面平整,具备良好的机械强度和硬度,杨氏模量和硬度分别为55GPa,2.5GPa。薄膜透光率与组装层数之间表现出良好的线性相关性。(2)探索了石墨烯/聚苯胺复合材料制备新方法。利用氧化还原反应原理,选择以氧化石墨烯、苯胺为原料、PW1z做催化剂,在紫外光激励下实现了石墨烯/聚苯胺复合材料的制备,通过改变苯胺单体浓度成功调控材料形貌结构。当苯胺单体浓度大于或等于3mmol/L时形成直径数百纳米、均长20μm聚苯胺纳米棒；苯胺浓度介于0.25～1mmol/L之间时形成直径约200nm～1000nm纳米颗粒。(3)基于微纳加工原理,成功实现了薄膜图案化设计与加工。利用体外细胞实验对薄膜生物相容性进行了初步探讨,研究显示功能薄膜对HUVEC细胞无毒性作用；共培养HepG2细胞生长形态完整、尺寸均匀,薄膜具备良好的生物相容性。更多还原

【Abstract】 As the basic structural unit of various allotropes of carbon,graphene hasattracted wide interest of scientists in the fields of science and engineering owing toexcellent electrical, optical, thermal, mechanical properties. In this paper, apatterned functional nanofilms was fabricated by LBL and nanoimprint techniquesusing graphene-based materials and co-cultured with HepG₂cells to confirm thebiocompatibility of the nanofilms.The main achievements include:（1） PSS-stabilized graphene sol wassuccessfully prepared by Hummer’s method. PSS-stabilized graphene and PAH werethen self-assembled alternatively onto the ITO glass, slides, quartz and siliconsubstrates to constructed （PEI/PSS）2（PAH/PSS-G）100self-supporting multilayersnanocomposite films with layered structures by cross-section SEM images. Thenanocomposite film with average thickness and roughness of approximately90nmand5nm exhibited good mechanical strength and hardness, Young’s modulus andhardness were55GPa and2.5GPa respectively.（2） Novel UV light-aided catalytical synthesis method was developed toprepare graphene/polyaniline composites; and mophology of the gaphene/PANicomposites was regulated successfully via changing the concentration of anilinemonomers. When aniline comcentration wasn’t less than3mmol/L, nanorods withhundreds of nanometers in diameter,20m in length were obtained, otherwisenanoparticles with a diameter of about200nm¹000nm were formed.（3） Based on miro/nano processing principles, patterns were fabricated in thefilms, and then biocompatibility was discussed through HUVEC cells toxicity testsin vitro and co-culturing with HepG₂cells, which showed that the founctional filmwas no obvious toxic to HUVEC cells and was convenient for HepG₂cells culturing.更多还原