【作者】 李虎；

【导师】 杨开伦；

【作者基本信息】 新疆农业大学 ， 动物营养与饲料科学， 2013， 硕士

【摘要】 抗运动氧化应激是近年来生物科学领域的研究热点之一。血浆中丙二醛（MDA）浓度被广泛用于评价动物机体的抗氧化能力指标，但有关血浆中MDA浓度与动物机体组织中MDA含量之间的关系报道极少。本研究以SD大鼠为研究对象，分别研究相同运动时间模型和力竭模型下，灌服不同剂量硫辛酸对SD大鼠抗氧化能力的影响，及灌服不同剂量番茄红素对相同运动时间模型下SD大鼠抗氧化能力的影响，并对血浆中MDA浓度与动物机体组织中MDA含量之间的关系进行研究，证实测定血浆中MDA浓度以反映动物机体内发生氧化程度的可行性。灌服硫辛酸在相同运动时间模型时SD大鼠组织总MDA含量与血浆MDA浓度的拟合方程为Y＝0.3733X－2.546（8R2=0.9969）；硫辛酸剂量与血浆、组织总MDA含量的拟合方程分别为Y=﹣0.01X＋3.3767（R2=0.9992），Y＝﹣0.0268X＋15.868（R2=0.9993）。灌服硫辛酸显著提高了相同运动时间模型SD大鼠肝脏、心脏中的T-AOC，心脏、腓肠肌组织中的GSH-px活力；对降低SD大鼠各组织及血浆中MDA作用不显著，剂量作用不明显。灌服硫辛酸在力竭模型时SD大鼠组织总MDA含量与血浆中MDA浓度的拟合方程为Y＝0.4983X－2.0568（R2=0.8191）；灌服硫辛酸的剂量与组织总MDA含量的拟合方程为Y＝﹣0.0637X+12.278（R2=0.9957）。灌服硫辛酸显著提高了力竭模型时SD大鼠肝脏、肾脏、心脏、脑组织中的T-AOC，且40mg/kg的剂量组提高幅度最显著；显著降低SD大鼠心脏、肾脏组织及总组织和血浆中MDA浓度，20mg/kg组与40mg/kg组之间差异不显著。灌服番茄红素在相同运动时间模型时SD大鼠组织总MDA含量与血浆中MDA浓度的拟合方程为Y＝0.1049X+1.9676（R2=0.8290）。灌服番茄红素显著提高了相同运动时间模型下SD大鼠肝脏中的T-AOC，肾脏、脑组织中的GSH-px活力，脑组织、腓肠肌组织中的CAT活力，且20mg/kg的剂量组可达到较高水平；可显著降低各组织、组织中总MDA含量，血浆中的MDA浓度也随之显著下降，20mg/kg与40mg/kg组之间差异不显著。本研究表明，血浆中MDA浓度能够较好的反映机体MDA含量。不同运动模型灌服硫辛酸对改善机体抗氧化能力的作用不尽一致，对力竭运动机体抗氧化能力的改善作用更明显。相同剂量相同运动模型时，灌服番茄红素改善机体抗氧化能力的幅度高于硫辛酸。更多还原

【Abstract】 Resistance to oxidative stress induced by exercise is one of research focuses in biological sciences inrecent years. The plasma malondialdehyde (MDA) content is widely used to evaluate the anti-oxidantability of animal, but the relationship of the MDA content between plasma and animal tissues was reportedrarely. In this study, the same time model and exhaustive model were set to obverse the effects ofantioxidant capacity of SD rats with intragastric supplemental different doses of lipoic acid, and intragastricsupplemental with the different doses of lycopene on same time model of SD rats anti-oxidant capacity.The relationship of the content of MDA between plasma and animal tissues was also studied to confirm thatMDA content in plasma could be used to reflect oxidation degree occurred in animal organism.The content of MDA in tissue and the concentration of MDA in plasma of SD rats in same timeexerxise model intragastrised with lipoic acid presented linear relationship, linear fitting curve equationwas Y＝0.3733X－2.5468（R2=0.9969）. The dose of intragastric lipoic acid relationship between MDAcontent in plasma and total tissue was Y=﹣0.01X＋3.3767（R2=0.9992）and Y＝﹣0.0268X＋15.868（R2=0.9993）, respectively. T-AOC in liver，heart and GSH-px activity were increased significantly ofSD rats that at the same time model with intragastric lipoic acid, but the effect of decreasing MDA was notsignificant.The content of MDA in tissue and concentration of MDA in plasma of SD rats in exhausting timeexercise model intragastrised with lipoic acid presented linear relationship. Linear fitting curve equationwas Y＝0.4983X－2.0568（R2=0.8191）. The dose of intragastric lipoic acid relationship between MDAcontent in plasma and total tissue was Y＝﹣0.0637X+12.278（R2=0.9957）. T-AOC in liver, kidney, Heartand Brain tissue was increased with intragastrition of lipoic acid and was significantly improvedintragastrition with40mg/kg·BW. MDA concertration in heart, kidney, total tissue and plasma of SD ratsintragastrised with lipoic acid were significantly dcreased in exhausting model. There was not significantbetween animal intragastrised with lipoic acid20mg/kg and40mg/kg.The content of MDA in tissue and the concentration of MDA in plasma of SD rats in same timeexercise model with intragastric lycopene presented linear relationship, linear fitting curve equation was Y＝0.1049X+1.9676（R2=0.8290）, T-AOC of liver, GSH-px activity of kidney and brain tissue, CAT activityof brain and gastrocnemius tissue were all increased SD rats that at the same time model with intragastriclycopene，and intragastric with20mg/kg reached higher livel. MDA content in tissues and total tissue weresignificantly decreased, and plasma MDA concertration was also decreased. There was not significantbetween animal intragastrised with lycopene20mg/kg and40mg/kg.It was showed that MDA concentration of plasma could be confortable for reflecting MDA content intissues of animal. The effect of intragastric with lipoic acid on improvement of antioxidant ability in animaldepended on exercise models. Improvement of antioxidant ability was significant in exhaustion animal withadministration of lipoic acid. Antioxidant capacity of animal intragastrised with lycopene was better thanthat with lipoic acid in the same time exercise model.更多还原