【作者】 宋超；

【导师】 于红霞； 李凤琴；

【作者基本信息】 山东大学 ， 公共卫生（专业学位）， 2013， 硕士

【摘要】 目的：探讨黄芪提取物对小鼠辐照损伤的保护作用及抗氧化作用。方法：1.黄芪提取物抗辐射损伤作用将160只昆明种小鼠随机分为4组,每组又分为4个亚组,分别为黄芪提取物(受试物)低、中、高3个剂量组,剂量分别为150mg/kg.bw、00mg/kg.bw、900mg/kg.bw。另设辐照模型对照组,灌胃等量的蒸馏水,每组10只,每天灌胃1次,连续30天后,各组均以γ射线进行全身照射一次,根据检测指标不同,其中3个组照射剂量为4Gy,1个组照射剂量为7Gy,并继续给予受试物。辐照的前1天、后第3天和第14天进行外周血白细胞计数,辐照后第3天进行骨髓细胞DNA含量、骨髓细胞微核试验,辐照后第7天测定血红细胞及肝脏组织中超氧化物歧化酶(superoXide dismutase,SOD)活力。2.黄芪提取物抗氧化作用将昆明种小鼠40只随机分为模型对照组、低、中和高剂量组共4组,每组10只。三个剂量组每日分别灌胃给予150mg/kg.bw.300mg/kg.bw、900mg/kg.bw的受试物,模型对照组灌胃等量的蒸馏水。连续灌胃30天后,取尾血测定红细胞超氧化物歧化酶(superoXide dismutase,SOD)活力以及血液谷胱甘肽过氧化物酶(GSH-Px)活力。之后,各组小鼠均给予0.4mg/kg.bw的溴代苯油,20小时过后,处死小鼠,取肝脏组织,分别测定SOD活力、GSH-Px活力和丙二醛(MDA)的含量。结果：1黄芪提取物抗辐射损伤作用实验初期、中期、及实验结束时各组小鼠体重之间均无显著性差异(P>0.05)。照射前,辅照模型对照组与实验低、中、高剂量组的外周血白细胞数之间均没有显著性差异(P>0.05)。照射后第3天辐照模型对照组外周血白细胞数量较辐照前明显降低,差异有显著性(P<0.01),说明辐照损伤模型成立。照射后第14天,实验低剂量组和中剂量组与辐照模型对照组比较差异无显著性(P>0.05),高剂量组与辐照模型对照组比较,外周血白细胞数增高,差异有显著性(P<0.01)。照射后第3天,与辐照模型对照组比较,实验低、中、高剂量组骨髓细胞DNA水平均高于模型对照组,差异有显著性(P均<0.01)。与模型对照组比较,中剂量组、高剂量组的骨髓细胞微核率降低,差异具有显著性(P均<0.01)。而低剂量组小鼠的骨髓细胞微核率虽然低于辐照模型对照组,但是差异无显著性(P>0.05)。照后第7天,与辐照模型对照组比较,实验低、中、高剂量组的红细胞SOD活力均高于模型对照组,差异有显著性(P均<0.01,)。实验低、中、高剂量组小鼠肝脏组织中SOD活性显著性高于辐照模型对照组(P均<0.01)。2黄芪提取物抗氧化作用红细胞SOD活力测定结果显示,与模型对照组比较,低剂量组差异无显著性(P>0.05)、中剂量组、高剂量组小鼠红细胞SOD活力高于模型对照组,差异有显著性(P均<0.01)。实验低、中、高剂量组小鼠肝脏组织中SOD活力显著高于模型对照组(P均<0.01)。与模型对照组比较,实验低、中、高剂量组小鼠全血GSH-Px活力明显增强,差异均有显著性(P<0.05,P<0.05,P<0.01)。肝组织GSH-Px活力,低剂量组、中剂量组与模型对照组比较,差异无显著性(P>0.05),高剂量组与模型对照组比较,活性增加,差异有显著性(P<0.01)。肝组织中丙二醛(MDA)含量测定,低剂量组、中剂量组、高剂量组的丙二醛含量均低于氧化模型对照组比较,差异均有显著性(P<0.01)。结论：1黄芪提取物抗辐射损伤作用实验结果显示,给予黄芪提取物组小鼠外周血白细胞数量、骨髓细胞DNA水平、红细胞以及肝脏中SOD活性明显高于辐照模型对照组,骨髓细胞微核率明显低于辐照模型对照组,高剂量组的黄芪提取物显示出最强的抗辐射损伤功能,提示黄芪提取物对辐射损伤具有一定保护作用。2黄芪提取物抗氧化作用实验结果显示,给予黄芪提取物组小鼠的血液及肝脏组织中的超氧化物岐化酶活性、谷胱甘肽过氧化物酶活性明显高于模型对照组,而肝脏组织中丙二醛含量明显低于模型对照组,提示黄芪提取物具有一定的抗氧化作用。更多还原

【Abstract】 Objective:To study the protective effects on mice with irradiation damage and the anti-oxidation function of Astragalus extractsMethods:1The anti-radiation damage effects of Astragalus extracts160Kunming mice were randomly divided into4groups, each group was randomly divided into4subgroups, the Astragalus extracts low-dose group, moderate-dose group, high-dose group, and the model control group (10mice each group). The three dose groups were given Astragalus extracts at doses of150mg/kg.bw、300mg/kg.bw、900mg/kg.bw. The model control group was given distilled water.30days later,3groups were given the y-irradiation at the dose of4Gy, and1group7Gy. The total number of the white blood cells(WBC) in the peripheral blood was measured1day before radiation,3days and14days after radiation. The DNA content and the ratio of micronucleus cells in marrow were measured3days after the radiation The SOD activity in blood and liver were measured7days after radiation.2The anti-oxidation effects of Astragalus extracts.40Kunming mice were randomly divided into4groups, the model control group, the Astragalus extracts low-dose group, moderate-dose group, high-dose group. The three dose groups were given Astragalus extracts at doses of150mg/kg.bw、300mg/kg.bw、900mg/kg.bw. The model control group was given distilled water at the same dose.30days later, the SOD activity in erythrocytes and GSH-Px activity in blood were measured. Then0.4mg/kg.bw Bromobenzene was given.20hours later, mice were killed, then the SOD activity, GSH-Px activity and MDA content in liver were measured.Results:1The anti-radiation damage effects of Astragalus extracts At the first of the experiment, in the middle of the experiment,and at the end of the experiment, the body weight in each subgroup were not significantly different(P>0.05).1day before radiation, the model control group was compared with the low dose group, the moderate group and the high dose group, there was no significant difference(P>0.05).3days after radiation, WBC in the high dose group increased compared with the model control group(P<0.01), but there were no difference between low dose, high dose group and model control group(P>0.05). WBC of the model control group decreased significantly3days after radiation compared with1day before radiation(P<0.01),and it shows that the radiation model was successful.14days after radiation, the model control group was compared with the low dose group and moderate group, there were no significant difference(P>0.05). The WBC in high dose group increased significantly compared with model control group(P<0.01).3days after radiation were given, the DNA content in marrow of the model control group were higher compared with the three dose groups, and the difference were all significant(P<0.01).The ratio of micronucleus cells in marrow of model control group were higher compared with the three dose groups, and the difference were significant between moderate dose group, high dose group and model control group(P<0.01). The ratio of micronucleus cells in marrow of low dose group was not different with the model control group (P>0.05).7days after the y-irradiation were given, the erythrocytes SOD activity in the three dose groups were all significantly higher compared with the model control group(P<0.01).The liver SOD activity in the three dose groups were all significantly higher than the model control group(P<0.01).2The anti-oxidation effects of Astragalus extracts. The erythrocytes SOD activity in the high dose group and moderate dose group were higher compared with the model control group(P<0.01),and there was no difference between the loe dose group and model control group(P>0.05).The liver SOD activity in the three dose groups were all much higher compared with the model control group(P<0.01).The blood GSH-Px activity in low dose group, moderate dose group, and high dose group were all significantly higher compared with the model control group (P<0.05, P<0.05, P<0.01)。The liver GSH-Px activity in high dose group was significantly higher compared with the model control group(P<0.01),and there was no difference between low dose group,moderate group and the model control group(P>0.05).The MDA content in the low dose group, moderate group, and high dose group were significantly lower compared with the model control group((P<0.01)。Conclusions:1. The result of the anti-radiation damage experiment shows that the number of the white blood cells of the peripheral blood, DNA content level in marrow, the erythrocytes SOD activity and liver SOD activity in the groups given Astragalus extracts were much higher than the model control group, and the ratio of micronucleus cells in marrow was much lower than the model control group. The high dose group of Astragalus extracts have the strongest anti-radiation damage activity. It shows that the Astragalus extracts have protective effects on radiation damage.2. The result of the anti-oxidation experiment shows that the groups given Astragalus extracts have much higher erythrocytes SOD activity, liver SOD activity, blood GSH-Px activity, liver GSH-Px activity, and much lower MDA content in liver than the model control group. It shows that the Astragalus extracts have anti-oxidation function.更多还原