【作者】 王莹；

【导师】 李学德；

【作者基本信息】 安徽农业大学 ， 环境科学， 2012， 硕士

【摘要】 本文研究了一种简便有效的水体中磺胺嘧啶（SDZ）、磺胺二甲基嘧啶（SM2）、磺胺甲噁唑（SMZ）、磺胺氯哒嗪（SCP）、磺胺间二甲氧嘧啶（SDM）、四环素（TC）、土霉素（OTC）、金霉素（CTC）和强力霉素（DOC）的残留分析方法，评价了该方法的准确度和精密度；并进行了安徽部分饮用水源中9种抗生素的污染调查；检测分析了某市主要饮用水源保护区中抗生素的含量分布和季节变化特征以及该市污水处理厂进出水中的抗生素含量水平。主要研究结果如下：1.水样由1mol/L稀盐酸调节pH值后，通过OasisHLB固相萃取柱萃取富集，采用超高效液相色谱-串联质谱（UPLC-MS/MS）进行定量分析。9种目标抗生素的方法定量限（LOQ）为1.0~5.3ng/L。水样中目标抗生素在加标质量浓度分别为20ng/L、200ng/L和1000ng/L时的回收率均大于78.00%，相对标准偏差（RSD）低于10.18%，方法的准确度、精密度和灵敏度能满足实际样品检测要求。2.对安徽部分饮用水源中9种抗生素的污染状况进行调查：在自来水中检测到SMZ、SM2、SDM、DOC、TC、OTC和CTC，检出浓度介于1.71ng/L~21.92ng/L，其中SMZ和DOC的检出率最高，分别为59.1%和63.6%；在地下水中检测到SDZ、SMZ、SM2、SDM、DOC、TC、OTC和CTC，检出浓度介于1.53ng/L~20.56ng/L，其中SMZ和SM2的检出率最高，分别为64.3%和42.9%；在地表水中检测到SDZ、SMZ、SM2、SDM、DOC、TC、OTC和CTC，检出浓度介于1.54ng/L~78.74ng/L，其中SMZ和DOC的检出率最高，分别为81.25%和43.8%。3.初步探讨了某市主要饮用水源保护区中抗生素的分布及季节变化特征：SDZ、SMZ、SM2、SDM、DOC、TC、OTC和CTC在四个季节都有检出，含量范围在2.43ng/L~49.43ng/L；其中SMZ、SM2、TC、OTC和CTC有较高的检出率；秋季检出抗生素总量高于其他季节。4.检测了某市7个不同污水处理厂进出水中的抗生素含量水平：抗生素进出水的总浓度范围分别为73.7ng/L~248.99ng/L和34.51ng/L~166.29ng/L；SDZ、SMZ、SM2、SCP、DOC、TC、OTC和CTC在进水中有检出，浓度范围为2.13ng/L~205.76ng/L；SDZ、SMZ、SM2、DOC、OTC和CTC在出水中有检出，浓度范围为2.66ng/L~135.12ng/L。更多还原

【Abstract】 A simple and efficient residue analytical method was established for the analysis ofSDZ, SM2, SMZ, SCP, SDM, TC, DOC, OTC and CTC in water samples in this study, theaccuracy and precision of the method was investigated. The investigations of5SAs and4TCs residues in source of drinking water were carried out for parts of Anhui area. Thedistributions and seasonal change characteristics of9antibiotics in the main protected areafor water supply in a city were studied, and the occurrence and fate of9antibiotics werepreliminarily studied during the municipal sewage treatment plants in a city. The mainresults were summarized as following:1. Water samples were acidified and extracted through a Hydrophile-LipophileBalance (HLB) cartridge, quantitative analysis was carried out on the ultra pressure liquidchromatography coupled with tandem mass spectrometry (UPLC-MS/MS). The limits ofquantification were1.0~5.3ng/L for9antibiotics in water samples. The average recoveriesof9antibiotics from water matrices at three spiked levers of20ng/L,200ng/L and1000ng/L were above78%, with relative standard deviations below10.18%. All the resultsmeet the requirements of analysis and successfully used in the determination of antibioticdrugs in water samples.2. The pollution survey of9antibiotics in drinking water for parts of Anhui area:SMZ, SM2, SDM, DOC, TC, OTC and CTC have been detected in the tap water, atconcentrations ranged from1.71ng/L to21.92ng/L, especially, SMZ and DOC were themost frequently detected antibiotics, which have been detected in59.1%and63.6%ofwater samples respectively; SDZ, SMZ, SM2, SDM, DOC, TC, OTC and CTC have beendetected in the ground water at concentrations ranged from1.53ng/L to20.56ng/L,especially, SMZ and SM2were the most frequently detected antibiotics, which have beendetected in64.3%and42.9%respectively of water samples; SDZ, SMZ, SM2, SDM, DOC,TC, OTC and CTC have been detected in the surface water at concentrations ranged from1.54ng/L to78.74ng/L, SMZ and DOC were the most frequently detected antibiotics,which have been detected in81.25%and43.8%respectively of water samples.3. The distributions of9antibiotics and seasonal change characteristics in the mainprotected area for water supply in the city: SMZ、SM2、SDM、DOC、TC、OTC and CTChave been detected in four seasons, at concentrations ranged from2.43ng/L to49.43ng/L;SMZ、SM2、TC、OTC and CTC were the most frequently detected antibiotics, the total concentrations of autumn were higher than the other seasons.4. The occurrence and fate of the selected antibiotics were preliminarily studiedduring the municipal sewage treatment plants in the city. The concentrations in the raw andtreated sewage are73.7ng/L~248.99ng/L and34.51ng/L~166.29ng/L. SDZ、SMZ、SM2、SCP、DOC、TC、OTC and CTC have been detected in the raw sewage, with concentrationsranging from2.13ng/L to205.76ng/L. SDZ、SMZ、SM2、DOC、OTC and CTC have beendetected in the treated sewage, with concentrations ranged from2.66ng/L to135.12ng/L.更多还原