【作者】 张鹏；

【导师】 黄瑞松； 黄荣韶；

【作者基本信息】 广西大学 ， 植物学， 2012， 硕士

【摘要】 钩藤(Uncaria rhynchophylla (Miq.)Miq. ex Havil.)和毛钩藤(U.hirsuta Havil.)为茜草科钩藤属植物,其带钩茎枝均为《中国药典》收载的钩藤药材。主要含生物碱类成分,2010年版《中国药典》一部“钩藤”项下未收载含量测定和指纹图谱测定项,未能有效控制钩藤药材的质量；过去人们对钩藤属植物的不同种、不同产地、不同部位和不同采集时间的药材质量缺乏系统研究和了解。本课题以钩藤和毛钩藤为研究对象,在建立钩藤药材钩藤碱含量和指纹图谱的测定方法的基础上,对其不同种、不同产地、不同部位和不同采收期的样品进行钩藤碱含量及指纹图谱测定,并分析评价其药材质量,为广西钩藤属植物资源的开发利用提供试验依据。研究成果如下：1、建立了钩藤植物钩藤碱含量高效液相色谱(HPLC)测定方法和基于HPLC的指纹图谱测定方法。2、钩藤和毛钩藤植物所含钩藤碱成分的状况随着种和产地的不同差异较大,26批不同产地钩藤药材有11批可检出钩藤碱成分,检出钩藤碱样品占总批次42.3%,含量为0.014%～0.386%(以药材干燥品计算,下同)；9批毛钩藤样品均未检出钩藤碱成分,未检出钩藤碱样品占总批次的100%。3、含有钩藤碱的钩藤药材不同部位中钩藤碱的含量稍有差异。钩藤药材除容县(17号)和荔浦县(18号)的叶未能检出钩藤碱成分外,其余产地带钩茎枝、无茎枝钩、无钩茎枝、主杆和叶等各部位均可以检出钩藤碱,除叶的钩藤碱含量较低外,其他部位钩藤碱含量相近。其中,带钩茎枝平均含量为0.141%,无茎枝钩为0.139%,无钩茎枝为0.138%,主杆为0.179%,叶为0.023%。4、不同采收期的钩藤药材中钩藤碱含量有较大差异,在一年内的变化范围是0.137%～0.386%,其中含量最高为2月份(0.386%),其次为3月份(0.371%),含量最低为7月份(0.137%)。5、以喜树碱峰作为外插参照峰,建立了钩藤和毛钩藤药材标准指纹图谱,并对钩藤和毛钩藤不同产地进行了指纹图谱测定。广西不同产地钩藤样品之间的指纹图谱具有较好的一致性。含量较高的主要化学组分的含量差异不大,大部分主要化学成分含量相似,质量较稳定。更多还原

【Abstract】 U. rhynchophylla (Miq.)Miq. ex Havil. and U. hirsuta Havil. were Rubiaceae genus Uncaria, which branch with hamulus has been embodied in Pharmacopoeia of People’s Republic of China (2010edition). Alkaloid were their effective components. Content determination and fingerprint of Uncaria has not been embodied in Pharmacopoeia of People’s Republic of China (2010edition), so it can not controlled quality of Uncaria. System research of Uncaria was lack. U. rhynchophylla (Miq.)Miq. ex Havil. and U. hirsuta Havil. from different places, different parts and different collecting times were determined with rhynchophylline content and fingerprint by the methods we builded, which were used to analysis, evaluation and control the quality of Uncaria, and provided a reference to develop and utilize of Uncaria. Research achievements as follows:1. Content detamination and Fingerprint determination of HPLC was builded.2. There was great differences between different varieties and places.11batches in26of different places of U. rhynchophylla (Miq.)Miq. ex Havil. contained rhynchophylline, which account for42.3%, content between0.014%～0.386%, and there were not detected of rhynchophylline in U. hirsuta Havil. from9different places, which account for100%.3. There was some differences on different parys in U. rhynchophylla (Miq.)Miq. ex Havil. which contained rhynchophylline.Except the leaves from Rong county(No.17) and Lipu county(No.18) were not detected rhynchophylline, other parts from different places can detected. Besides leaves has lower content of rhynchophylline, which content was0.023%, the other parts were similar(branch with hamulus was0.141%, hamulus without branch was0.139%, branch without hamulus was0.138%and stem was0.179%). 4. The content of rhynchophylline in different collecting times had great differences, which were0.137%～0.386%in a year. Among them, February was highest, which content was0.386%, the second was March, which content was0.371%, and July was the lowest, which content was0.137%.5. The standard chromatographic fingerprint of U. rhynchophylla (Miq.)Miq. ex Havil. and U. hirsuta Havil. were created, with the camptothecin peak as the external reference peak. The samples from different places were determined. It showed that the consistency of the fingerprints from different palces were good. Main chemical components which contents were high had little differences, most of them were similar, and the quality was stable.更多还原