【作者】 金哲洙；

【导师】 金华；

【作者基本信息】 延边大学 ， 内科学， 2012， 硕士

【摘要】 目的：研究尿毒清颗粒对糖尿病肾病大鼠肾脏病变的保护作用和对结缔组织生长因子(CTGF)和转化生长因子-β1(TGF-β1)表达的影响。方法：对29只成年雄性Wistar大鼠,随机分为3组,正常对照组(NC,N=5)糖尿病肾病对照组(DN,N=12)、尿毒清组(N=12)。糖尿病肾病大鼠模型制作方法：腹腔注射链脲佐菌素(STZ,65mg/kg)建立糖尿病肾病模型。尿毒清组给予尿毒清(2.6g/kg/d)灌胃,正常对照组和DN对照组给予蒸馏水灌胃。在实验第4周、8周、12周末收集24小时尿液,检测24小时尿蛋白排泄量(24UPE)和尿肌酐(Ucr)；眼眶后静脉采血,测血糖(G1u)、血肌酐(Scr)、计算内生肌酐清除率(Ccr)。在实验第12周末处死全部大鼠,观察肾组织病理学变化,并采用免疫组化法观察肾组织中的结缔组织生长因子(CTGF)与转化生长因子-β1(TGF-β1)的表达水平。结果：①血糖：DN对照组、尿毒清组大鼠在12周后血糖仍明显高于正常对照组(P<0.01)。②DN对照组较正常对照组24UPE、Ccr显著升高(p<0.05)：尿毒清组与DN对照组比较,24UPE与Ccr明显下降,具有统计学意义(p<0.05)。③正常对照组大鼠CTGF和TGF-β1在肾小球和肾间质内无或极少表达。DN对照组CTGF和TGF-β1在肾小球和间质内有强烈表达(p<0.01),尿毒清组较DN组表达减轻(p<0.05)。④DN组的肾脏病理变化主要表现为肾小球体积增大、系膜基质增生,基底膜增厚等；与DN组相比较,尿毒清组的上述病理改变较轻。结论：①尿毒清可显著减少糖尿病肾病大鼠肾组织内CTGF和TGF-β1的表达。②尿毒清可延缓糖尿病肾病的进展。更多还原

【Abstract】 Objective:The protective effect of Niaoduqing particles on the renal lesions of diabetic nephropathy rats and the expression of connective tissue growth factor (CTGF) and transforming growth factor-beta (TGF-β1) on the renal lesions.Methods:29adult male Wistar rats were randomly divided into three groups, normal control group (NC, N=5), diabetic nephropathy in the control group (DN, N=12), Niaoduqing group (N=12). Diabetic nephropathy rat model of production methods: intraperitoneal injection of streptozotocin (STZ,65mg/kg), the establishment of a diabetic nephropathy model. Niaoduqing group was given the Niaoduqing (2.6g/kg/d) gavage normal control group and the DN in the control group given distilled water. In the four weeks of the experiment,8weeks,12weeks to collect24hours urine, to detect the24-hour urinary protein excretion (24UPE) and venous blood in the urine creatinine (Ucr);collecting orbital venous blood, measuring blood glucose (Glu), serum creatinine (Scr) and endogenous creatinine clearance rate calculation (Ccr). All rats were sacrificed in the12th week, the observed changes in the renal histopathology and immunohistochemical method to observe the renal tissue of the connective tissue growth factor (CTGF) and Transforming growth factor-beta (TGF-β1) expression levels.Results:①blood sugar:the DN in the control group, Niaoduqing group rats after12weeks, blood glucose was still significantly higher than the normal control group (P <0.01).②The DN control group compared with normal control group24UPE、Ccr significantly increased (p-<0.05); Niaoduqing group with DN control group comparison, these indicators decreased, with statistical significance (p-<0.05).③In DN group, CTGF and TGF-β1expression was significantly increased in region of glomerular and renal interstitial(p<0.01, compared with NC group); the increases were attenuation in Niaoduqing groups expression (p<0.05, respectively).④The DN group renal pathological changes in the main performance for the kidney small ball size increases, the mesangial matrix, basement membrane thickening; compared with the DN group, the pathological changes of, Niaoduqing group lighter.Conclusions:①Niaoduqing can significantly reduce the expression of CTGF and TGF-β1in renal tissue of diabetic nephropathy rats.②Niaoduqing can delay the progression of diabetic nephropathy.更多还原