【作者】 刘云峰；

【导师】 黄生高；

【作者基本信息】 中南大学 ， 口腔医学， 2012， 硕士

【摘要】 目的：探讨压应力对小鼠单核细胞RAW264.7分化过程中FcRy(Fc receptor I y chain)表达的影响。方法：以小鼠单核细胞RAW264.7为研究对象,设立实验组及对照组,在经巨噬细胞-集落刺激因子(macrophage colony-stimulating factor, M-CSF)和核因子κB受体活化因子配体(receptor activator of nuclear factor-KB ligand, RANKL)诱导培养4天后,采用SXG4201四点弯曲细胞力学加载仪,对实验组分别加载压应力0,3,6,12小时,以RT-PCR检测实验组及对照组FcRy mRNA的表达情况。结果：(1)小鼠单核细胞RAW264.7在破骨细胞培养液中培养后,其体积变大,核的数目增多,抗酒石酸酸性磷酸酶染色(TRAP)染色实验阳性；(2)当压应力刺激加载后,与对照组相比,实验组的FcRy mRNA表达量在第3小时轻微增加,但无统计学意义,自第6小时开始上调(P<0.05),且随加力的时间延长而增加(P<0.05)。结论：(1)压应力刺激使RAW264.7细胞FcRy mRNA的表达量上升；(2)在12小时范围内,FcRy mRNA的表达量随压应力的作用时间增加而逐渐上升,具有时间依赖性。更多还原

【Abstract】 Objective:To investigate the FcRymRNA expression of mouse monocytes RAW264.7subjected to compressive stress.Methods:We used mouse monocytes RAW264.7, and after osteoclasts were formed in conditioned culture medium, we subjected them to compressive stress using four-point bending system. After0,3,6,12hour’s time, we examined the expression of FcRy mRNA by RT-PCR.Results:The amount of RAW264.7cells increased, the volume of them enlarged, and the number of nuclei per osteoclast increased in vitro; The FcRy mRNA expression of RAW264.7cells subjected to compressive stress increased along with the time (P<0.05).Conclusions:Compressive stress can increase FcRy mRNA expression of osteoclasts. And this effect adds along with the time in12hours.更多还原