【作者】 杨敏；

【导师】 张幸平；

【作者基本信息】 重庆医科大学 ， 肿瘤学， 2012， 硕士

【摘要】 目的：观察EGFR单克隆抗体西妥昔单抗（Cetuximab）联合PTX对人乳腺癌MCF-7细胞的生长抑制作用。方法：实验分为对照组、Cetuximab组、PTX组、联合用药组。用MTT法检测Cetuximab、PTX及Cetuximab与PTX不同联合给药方案对MCF-7细胞的生长抑制率，流式细胞术检测各组细胞的凋亡率和周期分布，免疫荧光法及RT-PCR法检测PTX组和对照组细胞的EGFR蛋白及mRNA的表达。结果：MTT法检测发现PTX对MCF-7细胞的抑制作用呈剂量依赖性；Cetuximab与PTX不同的联合给药方案对MCF-7细胞的抑制强度不同，先予PTX再予Cetuximab可见明显的协同作用。与对照组相比，其余三组的细胞凋亡率均有所增加，但联合用药组较单药组更明显，差异有统计学意义（P<0.05）。与对照组比较，PTX组细胞的EGFR蛋白、mRNA的表达均减少（P<0.05）。结论：人乳腺癌MCF-7细胞对PTX敏感；PTX对MCF-7细胞的抑制作用呈剂量依赖性；Cetuximab和PTX联用可明显抑制乳腺癌MCF-7细胞的生长，促进其凋亡，二者联用具有协同作用。更多还原

【Abstract】 Objective: To observe the growth inhibition effect of Cetuximab combined with Paclitaxel on Human Breast Cancer Cell Line MCF-7.Methods:The experiment involved four groups:the control group, Cetuximab group,PTX group and the combination group. The inhibitory rates of Cetuximab,PTX and different combinations of Cetuximab and PTX on MCF-7cells were examined by MTT assay and the apoptosis rate and cell cycle distribution were analyzed by flow cytometry. The immunofluorescence and RT-PCR were used to evaluate the EGFR protein and mRNA expression in the control group and PTX group.Results: PTX can exert inhibitory effect on MCF-7cells in a dose-dependent manner based on the results from MTT assay. MTT assay showed that different combination schemes exerted different inhibitory effects; the synergistic effect was seen in the scheme of MCF-7cells first treated with PTX and then Cetuximab. The apoptosis rate of the other three groups was increased compared with that of the control group and the effect was more evident in the combination groups than in the single agent groups (P<0.05). The EGFR proteins and mRNA expressions were reduced in PTX group compared with those in the control group (P<0.05).Conclusion:Human breast cancer cell line MCF-7was sensitive to PTX and PTX can exert inhibitory effect on MCF-7cells in a dose-dependent manner. The combination of Cetuximab and PTX can increase the apoptosis and decrease the proliferation of breast cancer cell line MCF-7with a synergistic action.更多还原