【作者】 王军；

【导师】 韩树峰；

【作者基本信息】 山西医科大学 ， 外科学， 2012， 硕士

【摘要】 目的：观察大剂量甲基强的松龙(MP)联合MK-801(地卓西平马来酸盐)对大鼠急性脊髓损伤的治疗效果。方法：采用Allen的重物坠落法建立大鼠急性脊髓损伤模型,将72只致伤大鼠一次性随机分为4组,每组18只大鼠(其中每组又包括24h、72h、15天三个亚组,每个亚组6只大鼠)。对照组伤后0.5h腹腔注射5ml生理盐水；MP组伤后0.5h腹腔注射甲基强的松龙30mg/kg体重；MK-801组伤后0.5h腹腔注射2.5%MK-801(地卓西平马来酸盐)5mg/kg体重；联合组伤后0.5h腹腔注射甲基强的松龙和MK-801(剂量、浓度同前)。分别在伤后24h、72h、15天处死动物,观察受损部位脊髓的病理形态和细胞凋亡,其中15天组分别于第1、3、5、7、10、15天做Gales评分和斜板试验。结果：甲基强的松龙、MK-801及联合组治疗大鼠急性脊髓损伤,在Gales评分、斜板试验及病理形态上、细胞凋亡检测与对照组相比较有明显改善,三个治疗组间相互比较无明显差异。结论：根据本次试验可以得出如下结论：1、单独应用MK-801与单独应用甲基强的松龙对大鼠急性脊髓损伤的治疗作用无明显差异。2、MK-801与甲基强的松龙联合对急性脊髓损伤的治疗作用,与两药单独应用无明显差异。更多还原

【Abstract】 Objective:to observe the high-dose methyl-prednisone (MP) joint consultation MK-801(Dizocilpine Maleate) to rat acute spinal cord injury therapeutic effect.Methods:Allen’s sport in heavy falls method to establish the model of rats acute spinal cord injury,72only injury rat one-time randomly divided into4groups, and18rats (one for each group includes24h,72h,15days, three group, each group six rats). After the control group of0.5h injuried intraperitoneal injection5ml physiological saline, After the MP group of0.5h injuried intraperitoneal injection methyl-prednisone30mg/kg weight, After the MK-801group of0.5h injuried intraperitoneal injection2.5%MK-8015mg/kg weight Joint group after intraperitoneal injection of0.5h injuried methyl-prednisone and consultation MK-801(dose, concentration before). After the injury24h,72h, in15days animal respectively death, damage of the spinal cord and pathological cell apoptosis, including the first15days in group1,3,5,7,10,15days do Gales and the inclined plate test.Results:methyl-prednisone, MK-801and combined treatment group acute spinal cord injury in rats were compared with control group,, the inclined plate test. Gales scores and pathological morphology and apoptosis detection has greatly improved, but three treatment group in the above aspects are at the same level.Conclusion:methyl-prednisone, MK-801and the combination of the two drugs rats acute spinal cord injury therapeutic effect has not different efficacy obviously in the present experimental conditions.更多还原