【作者】 乔晓鸣；

【导师】 任世存；

【作者基本信息】 青海大学 ， 中西医结合临床， 2012， 硕士

【摘要】 目的：观察沙棘总黄酮对血管性痴呆模型大鼠的影响，探讨其部分机制。方法：Wistar大鼠，普通级、180±20g、雌雄各半。采用高脂饲料加改进的双侧颈总动脉永久结扎（2-VO）法制备高脂血症血管性痴呆大鼠模型。取造模存活大鼠随机分为假手术组、模型组、对照组(银杏叶提取物)、实验组（沙棘总黄酮高、中、低剂量组），按规定剂量灌胃给药，假手术组给予等量生理盐水。各组灌胃25天后，采用Morris水迷宫检测模型大鼠空间学习记忆能力；灌胃30天后，采血测血脂和血流变学指标，断头取脑，分离右侧大脑皮质，测脑组织SOD活力和MDA含量，ELISA法检测血清TNF-α、IL-1β。以上均比较各组间的差异。结果：1、Morris水迷宫：定位航行实验中，各组和假手术组相比，有统计学意义（P＜0.05）；对照组、实验组与模型组相比，有统计学意义（P＜0.05）。在空间探索实验中，各组和假手术组相比，有统计学意义（P＜0.05）；假手术组、对照组、实验组（中、高剂量组）与模型组相比，有统计学意义（P＜0.05）。2、血脂和血流变学：对照组、实验组与假手术组相比，有统计学意义（P＜0.05）；对照组、实验组与模型组相比，有统计学意义（P＜0.05）。3、脑组织MDA含量和SOD活力：模型组和假手术组相比，有统计学意义（P＜0.05）,提示模型组MDA含量升高，SOD活性降低；对照组、实验组与模型组相比，MDA含量有统计学意义（P＜0.05）；对照组、实验组（中、高剂量组）与模型组相比，SOD含量有统计学意义（P＜0.05）；实验高剂量组与对照组相比，有统计学意义（P＜0.05）。4、血清中炎症因子TNF-α、IL-1β：模型组和假手术组相比，有统计学意义（P＜0.05）,模型大鼠血清炎症因子TNF-α、IL-1β含量显著增加；与模型组相比有统计学意义（P＜0.05）。结论：沙棘总黄酮通过改善实验大鼠的行为学、血流动力学、增加抗氧化能力、减轻脑组织被自由基损害、有效抑制炎症因子等机制、对血管性痴呆模型大鼠具有较好的治疗作用。更多还原

【Abstract】 Objective：Observation of total flavonoids of Hippophae rhamnoides on rat model ofvascular dementia effect，To investigate the mechanism of.Methods：Wistar rats，general level、180±20g、male and female half and half. Using highfat feed and improved permanent bilateral common carotid artery ligation (2-VO)preparation of hyperlipidemia vascular dementia rat model. Take the molding survivingrats were randomly divided into sham operation group, model group, the control group(Ginkgo biloba),the experimental group (total flavonoids of Hippophae rhamnoides in,high, middle, low dose group), According to the prescribed dose gavage administration,sham operation group was given the same amount of physiological saline. Each groupgavage for25days, Using the Morris water maze test model of spatial learning andmemory in rats; Gastric lavage is full after30days, blood lipid and blood rheologymeasurement, to decapitate,take out brain, nd separate the right cerebral corteximmediately for measuring activity of SOD、content of MDA. ELISA method for thedetection of serum TNF-α、IL-1β. Above all compare the differences among the groups.Results：1: Morris water maze test: during place navigation test, each group and shamoperation group compared, statistical significance（P＜0.05）. The control group, theexperimental group compared with the model group, statistically significant (P＜0.05).During space navigation test，each group and sham operation group compared,statistical significance（P＜0.05）. Sham operation group, the control group, theexperimental group (middle,high dose group) compared with the model group,statistically significant (P＜0.05).2:Blood lipid and blood rheology: The control group, the experimental group comparedwith sham operation group，statistically significant (P＜0.05).The control group, theexperimental group compared with the model group, statistically significant (P＜0.05).3:Brain tissue MDA content and SOD activity:Model group and sham operation groupcompared, statistically significant (P＜0.05), Tips of model group increased the contentof MDA, SOD activity decreased. The control group, the experimental group comparedwith the model group, the content of MDA,statistical significance (P＜0.05). Thecontrol group,the experimental group (medium,high dose group) compared with the modelgroup, The content of SOD, statistical significance (P＜0.05). Experimental highdosage group compared with the control group, statistical significance (P＜0.05).4：Serum inflammatory factor TNF-α、IL-1β: Model group and sham operation group compared, statistical significance (P＜0.05), model rats serum inflammatory factorTNF-α、IL-1βcontent increased significantly. Compared with the model group, statisticalsignificance (P＜0.05).Conclusion：TFH by improving the experimental rats behavior, hemodynamics, increaseantioxidant capacity, reduce brain free radical damage, effectively inhibit theinflammatory factors and mechanism, on rat model of vascular dementia and has bettertherapeutic effect.更多还原