【作者】 李猛；

【导师】 丁建华；

【作者基本信息】 大连理工大学 ， 光学工程， 2012， 硕士

【摘要】 拉曼光谱是研究分子振动、转动的一种光谱方法,能够对物质结构进行精确的分析,被广泛应用在多项科学领域。伴随着表面增强拉曼技术的出现,对物质检测的灵敏度得到极大的提高,逐渐的被作为一种精确的技术对物质进行定性和定量分析。本文介绍了拉曼光谱的基本理论,对表面增强拉曼光谱进行较全面的分析并且对其发展应用过程做了详细的解说。借助时域有限差分的方法对增强物质在理论上进行了模拟计算,最后探索利用表面增强拉曼光谱的方法对滤纸血斑中的促甲状腺激素浓度进行定量检测的方法。使用表面增强拉曼光谱(SERSp)和偏最小二乘回归(PLSR)方法对新生儿促甲状腺激素(TSH)定量检测进行初步研究,利用新生儿足跟滤纸血斑水洗液的表面增强拉曼光谱(SERSp)和偏最小二乘回归(PLSR)处理数据方法定量检测TSH浓度,与时间分辨免疫荧光定量检测TSH结果进行比较,探讨" SERSp-PLSR方法”定量检测TSH的可行性。在数据预处理时运用改进的多项式拟合的方法进行基线校正,能够较好去除荧光和基线漂移对实验结果造成的误差；用725cm-1峰强度作为归一化标准,以各个样品拉曼峰强度与725cm-1峰强度的比值为测量其浓度的依据,使用PLSR方法进行拟合得到回归方程,通过回归方程来求得其他未知样品的TSH浓度。用该样品已知TSH浓度,考核和评定用"SERSp-PLSR’‘方法定量检测的果的相符程度。就目前资料所知,本次实验是第一次使用SERSp-PLSR方法对血液中TSH浓度进行定量检测,实验得到的结果能够在一定程度上与时间分辨免疫荧光定量检测结果相符在一定程度上证明此方法是可行的,具有潜在应用前景,可能为新生儿促甲状腺激素的定量检测提供另一条快速简便的检测途径。更多还原

【Abstract】 Raman spectroscopy is widely used in many fields of science and allows one to derive detailed and specific information on a molecular vibration and rotation. By using surface-enhanced Raman technology, material detection sensitivity are greatly improved and it gradually as a precise technique for qualitative and quantitative analysis of the material.This paper introduces the basic theory of Raman spectroscopy and elaborate the application and study progress of surface-enhanced Raman spectroscopy. With the finite difference time domain method for enhancing substances, in theory, simulation, and finally explore the use of surface enhanced Raman spectroscopy method of quantitative detection of thyroid stimulating hormone concentration in filter paper blood spots.Preliminary study on the quantitative determination of thyroid stimulating hormone (TSH) in newborn blood by Surface-Enhanced Raman Spectroscopy(SERSp)-Partial Least Squares Regression (PLSR)based approach. The date of TSH concentration which obtained by SERSp-PLSR compared with the date form time-resolved fluoroimmunoassay to validate the feasible of the SERSp-PLSR method. An improved polynomial fitting method was used for baseline correction, which could remove the error of result caused by the fluorescence and baseline drift, the intensity of725cm-1Raman peak was used as a normalization standard, the ratio of the intensity of other Raman peak with the intensity of725cm-1Raman peak were used for measuring its concentration. And use of partial least squares regression fitting regression equation, which was used to obtain the other unknown samples of TSH concentration. This experiment is the first use of SERSp for quantitative detection of TSH concentration in the blood, the experimental results consistent with the actual but still need further improvement. To some extent, this method is feasible, and also develop a quick and easy path for the quantitative detection of TSH concentration.更多还原