【作者】 吴娟；

【导师】 周先礼；

【作者基本信息】 西南交通大学 ， 微生物与生化药学， 2012， 硕士

【摘要】 藏角蒿(Incarvillea younghusbandii Sprague)为紫葳科角蒿属植物,多年生矮小草本,其藏文名为乌确玛尔甲,是中国的特有种植物,分布在西藏等地,生于海拔3500-5400m的高山地区。藏角蒿为我国藏、蒙、彝、傈僳等多个民族常用草药,有滋补强壮之功效,主治产后乳少,气血不足,头晕疲倦；性温,味甘淡。藏角蒿花的化学成分未见报道,本文首次对藏角蒿花的化学成分进行了研究。本文首次对藏角蒿花的挥发性成分进行了研究。采用水蒸气蒸馏法提取藏角蒿花的挥发性成分,用毛细管气相色谱—质谱联用技术(GC-MS)对其化学成分进行分析,以面积归—法测定各个成分的相对百分含量。共分离出24个峰,鉴定了其中23个化学成分,占挥发油总量的99.59%。其挥发油的主要成分为十六酸(31.66%)、二十三烷(15.76%)、9-己基十七烷(8.37%)、十四酸(7.96%)、2,15-十六烷二酮(7.20%)等,为藏角蒿的充分开发利用提供了科学依据。本文采用硅胶柱层析、Sephadex LH-20柱层析、大孔树脂柱层析、RP-C18柱层析等多种分离手段对藏角蒿花的非挥发性成分进行分离纯化,从中分离得到17个单体化合物,利用光谱法(H NMR、13C NMR、MS)对这17个化合物进行了结构鉴定,分别为：β-谷甾醇(1),胡萝卜苷(2),乌苏酸(3),rengyolone (4),芹菜素(5),木犀草素(6),槲皮素(7),calceolarioside A(8),2’-乙酰基毛蕊花糖苷(9),木犀草素-7-O-β-D-吡喃葡萄糖醛酸苷甲酯(10),管花苷B(11),芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷甲酯(12),毛蕊花糖苷(13),2-(4-羟基-3-甲氧基苯基)乙基-O-β-D-吡喃葡萄糖苷(14),红景天苷(15),rengyoside B (16),异毛蕊花糖苷(17)。17个化合物均首次从该植物花中分离得到。更多还原

【Abstract】 Incarvillea younghusbandii Sprague, a member of Incarvillea (Bignoniaceae), is sometimes perennial dwarf herbs and its Tibetan name is WuQueMaErJia. Incarvillea younghusbandii Sprague is the specie endemic to China, which is mainly distributed in Tibet and grows in the mountains from3500to5400meters. This herb has been used as traditional medicines in the areas of Tibetan, Mongolian, Yi, Li su. Incarvillea younghusbandii Sprague has the function of strengthening physical condition, which is widely used for treating lactation deficiency, blood inadequacy, dizziness and fatigue. The thesis studies on chemical constituents from the flowers of Incarvillea younghusbandii Sprague for the first time..The objective is to analyze the chemical components of the volatile oils from the flowers of Tibetan medicine Incarvillea younghusbandii Sprague. The volatile oils of Incarvillea younghusbandii Sprague were extracted by water steam distillation and its chemical components were separated and identified by GC-MS. The content of each component was determined by area normalizetion method. Twenty four peaks were separated and twenty three components were identified, which constituted about99.59%of the total volatile oils. The main compounds are hexadecanoic acid(31.66%), tricosane(15.76%),9-hexyl-heptadecane(8.37%),myristic acid(7.96%),2,15-hexadecanedio-ne(7.20%)The constituents were separated and purified with chromatographic methods and their structures were elucidated by spectroscopic methods(1H NMR、13C NMR、MS). Seventeen compounds were isolated and identified as β-sitosterol(1), β-daucosterol(2), ursolic acid(3), rengyolone(4), apigenin(5), luteotin(6), quercetin(7), calceolarioside A(8),2’-acetylacteoside (9), luteolin-7-O-β-D-glucuronide methyl ester(10), tubuloside B(11), apigenin-7-O-β-D-glucuronide methyl ester(12), acteoside(13),2-(4-hydroxy-3-methoxyphenyl)-ethyl-O-β-D-glucopyranoside(14), salidroside(15), rengyoside B(16), isoacteoside(17). Seventeen compounds were isolated from the flowers of this plant for the first time.更多还原