【作者】 刘亮；

【导师】 管洪庚；

【作者基本信息】 苏州大学 ， 普外科， 2012， 硕士

【摘要】 目的：探讨姜黄素及其联合塞来昔布(celecoxib)对人胃癌细胞株（SGC-7901）生长抑制作用及凋亡的影响，并测定二者作用下细胞周期分布情况，以及COX-2表达情况,同时探讨它们的作用机制。方法：以SGC-7901人胃癌细胞为研究对象，用不同浓度的姜黄素和塞来昔布分别及联合作用于细胞，用MTT法检测细胞增殖抑制率，流式细胞仪（FCM)检测处理后细胞周期和凋亡的变化，RT-PCR方法检COX-2mRNA的表达情况，免疫蛋白印记（Western Blot）检测COX-2蛋白的表达情况。结果：（1）MTT显示姜黄素和塞来昔布（celecoxib）对胃癌细胞SGC-7901均有抑制作用，其抑制作用具有药物依赖性，选用对胃癌细胞具有低抑制作用的塞来昔布（celecoxib）和姜黄素联用时能较大幅度增强姜黄素的抑制效果，试验组与对照组及各试验组之间比较均有统计学意义（p<0.05）。（2）流式细胞仪检测结果示，联合组G0/G1期细胞比例较单用姜黄素或塞来昔布（celecoxib）组明显增加，S期和G2/M期比例明显减小，且联合组的凋亡率与姜黄素和单用塞来昔布组比较有明显的差异，其差异性有统计学意义（P<0.05）。（3）RT-PCR和Western Blot法结果表明，姜黄素或塞来昔布及联合组，COX-2表达明显减少，与对照组比较差异有统计学意义(P<0.05)，联合组较单药组有统计学意义(P<0.05)。结论：姜黄素对体外培养的胃癌细胞SGC-7901有抑制作用，并能诱导其凋亡，姜黄素联合塞来昔布对体外培养的胃癌细胞SGC-7901的生长有协同抑制效应，两药合用诱导胃癌细胞SGC-7901的凋亡作用显著强于单药组。此实验为姜黄素联合选择性COX-2抑制剂在肿瘤的化学预防中提供了理论依据。更多还原

【Abstract】 Objective: To investigate the effects of curcumin with celecoxib,a selective COX-2inhibitor, on cell proliferation and apoptos,COX-2Expression in SGC-7901human gastriccancer cell line and to explore its anti-neoplasm mechanism.Methods：MTT assay was used to determine cell proliferation after incubation indifferent concentrations of curcumin with celecoxib，The effect of curcumin with celecoxibon cell cycle changes and apoptosis of cells was studied by flow cytometry(FCM)，themRNA expressions of COX-2were analyzed by RT-PCR; Western Blot was used to detectCOX-2protein expression.Results:(1) MTT showed：curcumin with celecoxib on gastric cancer SGC-7901cells was inhibited, the inhibition increased with increasing doses of the drug, the choice ofno significant effects on cells of low doses of curcumin with celecoxib combined withsignificantly enhanced when compared with the inhibitory effect of curcumin。There weregreat statistical signification between medicated group each other (P<0.05).(2) Flowcytometry results suggest that the combination group G0/G1phase cells in comparisonwith curcumin or celecoxib group was significantly increased, S phase and G2/M phasewas significantly reduced (P<0.05).(3) RT-PCR and Western Blot results showed thatcurcumin or celecoxib and the combination group,COX-2expression was significantlyreduced compared with the control group significantly (P <0.05), combination groupcompared with single drug group Statistically significant (P<0.05)。Conclusion： Curcumin could inhibit the growth of human SGC-7901Gastricadenocarcinoma cells, which enhance desensitivity of curcumin on SGC-7901cells.Thecureumin and celecoxib can all induce human SGC-7901gastric Adenocarcinoma cellsapoptosis. When the two drugs combined，the inductive effect for cell apoptosis becamemore obvious. which may provide a theoretical basis for their use in chemoprevention of gastric cancel．更多还原