【作者】 王丽杰；

【导师】 董宇翔；

【作者基本信息】 吉林大学 ， 中西医结合临床， 2012， 硕士

【摘要】 目的：观察脑心康Ⅱ号大、中、小剂量对垂体后叶素(PIT)所致急性心肌缺血大鼠血清肌钙蛋白（cTnI）、肌红蛋白（Mb）及心功能左室射血分数（LVEF）、短轴缩短率（FS）左室舒张末内径(LVDD)、左室收缩末内径（LVDS）的影响，进一步探讨其作用机制，为临床心肌保护的中药运用提供实验依据。方法：选择8-10周龄健康清洁级Wistar大鼠60只随机分成6组：空白组（正常对照组）、阴性对照组（生理盐水组）、阳性对照组（丹参滴丸组）、脑心康II号大、中、小剂量组。各组大鼠饲养在于同一生活环境中，统一喂养固体动物饲料，正常进食水，给予相应液体灌胃20天后，对大鼠进行两次腹腔注射垂体后叶素（PIT）处置，间隔24小时，制备急性心肌缺血大鼠模型。第二次注射垂体后叶素30分钟后，给大鼠腹腔注射1.5%戊巴比妥钠（30mg/kg）麻醉成功后进行心电图检查，对心电结果符合造模成功标准的大鼠行心脏彩超检测，测得大鼠心功能左室射血分数（LVEF）、短轴缩短率（FS）左室舒张末内径(LVDD)、左室收缩末内径（LVDS）。第二次注射垂体后叶素3小时后采血，行血清肌钙蛋白（cTnI）、肌红蛋白（MB）含量测定。结果：脑心康II号大、中剂量组对垂体后叶素（PIT）引起的心肌缺血大鼠cTnI、Mb的升高幅度明显低于阴性对照组，心功能四项测得值明显优于阴性对照组，差异有统计学意义(P<0．05)，其中大剂量组差异显著(P<0．01)，小剂量组效果不明显（P＞O．05）。结论：脑心康II号对大鼠心肌具有保护作用，其机制可能与增加冠脉血流量，改善心肌供氧，促进血液循环，保护血管内膜，减少血小板聚集，提高心肌酶活性，减少心肌细胞缺血缺氧损伤及增强心功能有关。更多还原

【Abstract】 Objective: To observe Impact of Nao Xin Kang II (NXKIl) large, medium,small doseson pituitrin (PIT) due to acute myocardial ischemia in rat serum troponin I (cTnI),myoglobin (Mb), and cardiac function in left ventricular shoot The impact of ejectionfraction (LVEF), fractional shortening (FS), left ventricular diastolic diameter (LVDD) at theend, left ventricular end systolic diameter (LVDS), and further explore its mechanism, toprovide experimental basis for the use of Chinese medicine clinical on myocardial protection.Method:8-10weeks of healthy Wistar rats of clean grade,60were randomly divided into sixgroups: blank control group, negative control group, positive control group, NXKIl, large,medium and small dose group. Rats in each group in the same living environment, a unifiedfeeding solid animal feed, all groups were given liquid gavage for20days except the blankgroup, Rats were injected twice Pituitrin (PIT) Interval of24hours, Preparation acute ratmodel of myocardial ischemia. after the second injection of Pituitrin30minutes, Inspection theECG of rats who were Intraperitoneal injected with1.5%sodium pentobarbital (30mg/kg),after theanesthesia success of,detect the rats Echocardiography On the ECG results in line withsuccessful modeling standards,, measuring left ventricular ejection fraction (LVEF), cardiacfunction, fractional shortening (FS), left ventricular end diastolic diameter (LVDD), leftventricular end-systolic diameter (LVDS). After the second injection of Pituitrin three hours,Blood to detect serum troponin I (cTnI), myoglobin (MB) content, For acute myocardial ischemia ratscaused by Pituitrin (PIT), The cTnI, Mb increased range of NXKIl the large,middle dose group weresignificantly lower than the negative control group, Four measurements results of heart functionwere significantly better than the negative control group,the difference has statisticallymeaning (P <0.05), high-dose group was significantly different (P <0.01), low-dose group,the effect has not significant (P>0.05). Conclusion: NXKIl on myocardial protective effectand its mechanism may be associated with increased coronary blood flow, improvemyocardial oxygen supply, promote blood circulation, protect the vascular intima, reduceplatelet aggregation, improve the myocardial enzyme activity, reduction of myocardial cellinjury by hypoxia and enhanced cardiac function.更多还原