【作者】 孙伟；

【导师】 孙传洋；

【作者基本信息】 苏州大学 ， 泌尿外科学， 2011， 硕士

【摘要】 目的:研究GST-π、VEGF在肾癌及其癌旁组织中的表达及其与肾组织癌变的关系,探讨两者在肾组织癌变中的作用机制及生物学行为。方法:收集48例手术切除的肾癌标本作为实验组,另取45例癌旁标本作为对照组,采用免疫组化和逆转录聚合酶链反应方法在蛋白水平和基因水平,检测48例肾癌组织和45例癌旁正常肾组织中GST-π、VEGF的表达,并采集临床资料,分析其表达水平与肾组织癌变临床病理特征之间的关系。结果:l、GST-π蛋白在肾癌中的表达(41/48)高于癌旁正常肾组织(15/45),差异有统计学意义(P<O.05);VEGF蛋白在肾癌中的表达(33/48)高于癌旁正常肾组织(5/45),差异有统计学意义(P<0.05)。VEGF和GST-π蛋白在肾癌组织中的表达与患者性别、年龄、组织学分级及肿瘤大小无相关性,其中VEGF与肿瘤有无淋巴结转移等因素有明显相关性(P<0.01)。2、肾癌组织与癌旁正常肾组织相比,GST-Π、VEGF基因mRNA的表达增加(P<O.05)。结论:VEGF、GST-π在肾癌组织中均有不同程度的高表达;肾癌的侵袭性与原发性多药耐药可能与VEGF、GST-π有关。更多还原

【Abstract】 Objective: To investigate the expression of the glutathione S-transferase-π(GST-π) and vascular endothelial growth factor(VEGF) in renal carcinoma and para-tumor kidney tissue,and the factors influence the tumorigenesis,progression and biological behaviour.Methods: The expressions of VEGF and GST-πin 48 cases of renal carcinoma were analyzed by immunohistochemistry and RT-PCR, while 45 cases of para-tumor kidney tissue were served as the control group. The correlation between the expression of GST-π,VEGF and clinicopathological parameters was analyzed.Result: (1)The GST-πwas expressed in 41/48 renal carcinoma samples and 15/45 para-tumor kidney tissue samples.VEGF was expressed in 33/48 renal carcinoma samples and 5/45 para-tumor kidney tissue samples.There was no statistically significant correlation between GST-πor VEGF expression and gender, age, staging, (P>0.05).(2) The transcription of GST-πmRNA or VEGFmRN in renal carcinoma was higher than those in para-tumor kidney tissue samples(P<0.05).Conclusion: GST-πand VEGF are over-expressed in various degrees in renal carcinoma without chemotherapy, and they may be related to the primary multidrug resistance and aggressiveness of the tumors.更多还原