【作者】 吴永宏；

【导师】 刘新星；

【作者基本信息】 中南大学 ， 微生物学， 2011， 硕士

【摘要】 生物冶金具有成本低、流程短、环境友好等优势,目前在国内外得到了广泛的应用。永平铜矿是一个以铜硫矿物为主的大型综合性矽卡岩型矿床,1985年达到10000/d生产规模以来,产生了大量低品位硫化铜矿。已有研究表明常温菌对永平低品位硫化铜矿的浸出效果不佳,本文利用中温、中高温菌对永平铜矿进行处理,并研究了其浸出行为。永平低品位铜矿石品位为0.32,原生硫化铜矿占总铜量65.63%,根据黄铜矿和黄铁矿伴生的特点,选取6种典型中温、中高温浸矿菌种,按铁氧化功能菌和硫氧化功能菌相协同浸矿的原则,得到4组混合菌,进行共培养,对永平铜矿石进行浸出,以铜浸出率为首要的选择指标。结果表明,45℃C条件下嗜热铁质菌加嗜热硫氧化硫化杆菌加喜温嗜酸硫杆菌的组合浸出体系相对稳定,生物量持续生长,比其他混合菌组合浸出率高,为浸出永平低品位铜矿石的最佳组合。设计正交试验,对(嗜热铁质菌+嗜热硫氧化硫化杆菌+喜温嗜酸硫杆菌)混合菌的浸出条件进行优化,然后对正交实验结果进行验证并对中高温混合菌的浸出行为进行研究。结果表明永平低品位铜矿石浸出形成黄铜矿-黄铁矿原电池反应,浸出以间接作用为主,矿浆浓度对浸出率的影响最大,在最佳条件下(温度45℃、矿浆浓度15w/v、转速190r/min、和初始pH 2.0),摇瓶浸出6天浸出率达到94.26%。在摇瓶实验基础上,还设计了2L的搅拌槽进行周期为6天浸矿实验,摸索探讨槽浸法处理永平低品位铜矿石可行性,并对其浸出行为进行研究。更多还原

【Abstract】 Bioleaching technology has been used broadly around the world for its advantages, such as low cost, simple process and environmentally friendly. Yongping copper mine is a large skarn deposit. A large mass of copper-sulfide ore has accumulated since 1985 when the capacity reached 1000 t/d. A lot of researches have revealed that the leaching rate of copper sulfide ore was not good when using Mesophiles only. This study investigate the leaching reaction of Yongping low grade copper ore utilizing Mesophiles and Moderate thermophiles.The copper grade of Yongping copper ore is only 0.32%, and 65.63%of total copper minerals is the primary copper sulfide ore. According to the principle that ferrous oxidizing bacteria and sulfur oxidizing bacteria can cooperate in leaching reaction,4 groups of mixed bacteria were used in this study, and the leaching rate was set as criteria to screen the mixed bacteria. The leaching result showed that the combination of Ferroplasma thermophilium L1, Acidithiobacillus caldus S2 and Sulfobacillus thermosulfidooxidans YN22 under 45℃was the best group.And then, a series of orthogonal tests in shake flasks were performed to optimize the bioleaching conditions of the mixed culture Ferroplasma thermophilium L1, Acidithiobacillus caldus S2 and Sulfobacillus thermosulfidooxidans YN22. The results showed that the leaching rate of copper could reach to 94.26%were applied after 6 days, under optimized conditions of temperature 45℃、pulp density 15 w/v、rotational speed 190 r/min and initial pH 2.0. From the parameter drawn from this experiment, we can get the conclusion that the leaching mechanism is indirect interaction, and galvanic cell action was taken place between chalcopyrite and pyrite.An agitator tank was induced in this research. A 6 days’experiment was carried out, and the leaching behavior was studied. From that we can examine the practicability of battering method.更多还原