【作者】 杨光；

【导师】 王加启； 雒秋江；

【作者基本信息】 新疆农业大学 ， 动物营养与饲料科学， 2010， 硕士

【摘要】 试验Ⅰ:试验研究了十二指肠灌注富含α-亚麻酸（α-linolenic acid,LNA）的游离脂肪酸对泌乳奶牛免疫功能的影响。选择4头初产、健康且装有永久性十二指肠瘘管的中国荷斯坦奶牛进行2处理的交叉试验设计,用于灌注的脂肪酸设五个剂量,分别为:0、100、200、300、400 g/d,对照组只灌注乳化剂载体。采集血液样品,测定了奶牛血清中IgA,IgG,IgM,前列腺素E2（prostaglandin E₂, PGE2）和Th1/Th2细胞因子的含量。结果表明十二指肠增量灌注LNA线性增加了血清IgG含量,二次增加血清中干扰素-γ含量（P < 0.05）,而PGE2的浓度线性降低,白细胞介素（IL）-4趋于降低（P = 0.08）。LNA灌注对血清抗体IgA、IgM和细胞因子IL-2、IL-6、IL-10没有显著性影响。结论:α-亚麻酸的瘤胃后灌注通过影响幼稚T淋巴细胞及Th1/Th2型细胞因子的分泌从而起到调节免疫的功能。试验Ⅱ:通过十二指肠回撤灌注试验,研究四种不同十八碳脂肪酸（C18:0、C18:1、C18:2和C18:3 ）对泌乳奶牛免疫功能的影响。测定了奶牛血清免疫球蛋白（immunoglobulin, Ig）含量、外周血液T淋巴细胞亚群比例、血液中白细胞计数和体温变化。结果表明灌注十八碳脂肪酸对奶牛血液中Ig含量和体温影响不显著,C18:2的灌注有增加CD4+T细胞百分数的趋势,C18:3和C18:2的灌注显著降低血液中白细胞数量,C18:1的灌注有增加血液中单核细胞的趋势。C18:3和C18:2的十二指肠灌注具有增强和调节泌乳奶牛免疫力的功效。更多还原

【Abstract】 Trial one: Objective was to determine the effects of duodenal infusion of a highα-linolenic acid (LNA) on the immune function of lactating dairy cows. Four primiparous lactating Chinese Holstein cows fitted with duodenal cannula were administered 2 treatments in a crossover design: rich-α-linolenic acid infusion at varying concentrations (0, 100, 200, 300, and 400 g/d) vs. basal infusate control. Blood was collected and serum IgA, IgG, IgM, prostaglandin E2 (PGE2) and Th1/Th2 cytokines were determined. Results showed that increasing the supply of LNA to the small intestine of dairy cows linearly increased serum IgG and quadratically enhanced interferon-γ(P < 0.05), whereas the concentrations of PGE2 declined linearly (P < 0.05) and that of interleukin (IL)-4 tended to decrease (P = 0.08). No difference was observed in serum IgA, IgM and other cytokines, such as IL-2, IL-6 and IL-10. This study demonstrated that post-ruminal infusion of LNA affected the na?ve T lymphocytes and modified the secrection of Th1/Th2 cytokines, which showed the immunomodulatory properties of LNA.Trial two: Evaluated effects of duodenal infusion of fatty acids with 18 C (18-C FA; C18:0, C18:1, C18:2 and C18:3) on immunity of dairy cows. The total concentration of immunoglobulin (Ig) in serum, T lymphocyte subsets in peripheral blood, the acount of white blood cell (WBC) and body temperature were determined. Duodenal infusion of 18-C FA did not affect Ig in serum and body temperature. Duodenal infusion of C18:2 tended to increase the amount of CD4+T lymphocyte. Duodenal infusion of C18:3 and C18:2 decreased the acount of WBC significantly. Duodenal infusion of C18:1 tended to increase the acount of monocyte. Duodenal infusion of C18:3 and C18:2 increase and regulate immunity of dairy cows.更多还原