【作者】 李望；

【导师】 崔国贤；

【作者基本信息】 湖南农业大学 ， 作物栽培学与耕作学， 2009， 硕士

【摘要】 本研究在湖南农业大学苎麻研究所实验苗圃地,采用膨胀珍珠岩盆栽试验与大田抽样实验相结合,对25个栽培品种进行了高绿原酸含量种质资源的筛选工作,对苎麻绿原酸检测方法进行了较系统的比较,并对苎麻绿原酸提取检测工艺进行了优化,主要的研究结果如下:1.紫外分光光度法检测绿原酸的回收率为101.12%,cv=0.51%,相关系数为0.9979;液相色谱法的RSD=1.45%,相关系数为0.9998,说明两种方法均能用于苎麻绿原酸的检测工作,但由于液相色谱法费用昂贵,损耗过大,目前只用于实验室的精密检测阶段,紫外分光光度法适应于批量检测。2.对绿原酸的稳定性进行了研究,结果表明在室温、24h内较稳定。在碱性条件较易分解,在pH=4的酸性条件下较稳定,绿原酸在强光照射条件下容易分解。因此在绿原酸提取分离中尽量保持在酸性、避光条件下进行。3.最佳的苎麻样品干燥方式是:先置于通风阴凉处放置24h,再置于60℃恒温干燥箱中烘48h至恒重;绿原酸的最适提取溶剂为70%的乙醇;通过单因素实验分析得出绿原酸提取的最佳工艺参数为:浸提时间为2小时,温度为70℃,pH值为4.0,料液比为1:10。通过正交实验分析得出,各因素对苎麻叶中绿原酸提取率的影响由大到小的顺序为:浸提温度、浸提时间、浸提液pH值、料液比。4.对25个参选苎麻品种进行了田间农艺性状调查,并检查其叶片的绿原酸含量,其含量范围在0.731%-0.059%之间,依此初步得到了6个绿原酸含量比较高的品种,含量最高的是湘苎一号,是安仁黄水麻绿原酸含量的12倍。苎麻绿原酸含量与其株高的相关性(R~2=0.0155)、茎粗的相关性(R~2=0.0023)均不显著。5.通过小区实验,得知苎麻不同部位、不同季节、不同麻龄的绿原酸含量也存在差异:各部位中以根的平均含量最高,为0.538%,叶片次之,为0.528%;三麻叶的含量(为0.528%)高于头麻(为0.191%)和二麻(为0.353%);新栽麻麻蔸的绿原酸含量(为0.538%)高于二龄麻(为0.482%)和四龄麻(为0.340%)的含量。更多还原

【Abstract】 This study in Hunan Agricultural University,Institute of Experimental Nursery Ramie,the use of expanded perlite pot experiment with a combination of Daejeon experimental sample of 25 cultivars had a high content of chlorogenic acid in the screening of germplasm resources of ramie chlorogenic acid detection methods of a more systematic comparison,ramie and detection of chlorogenic acid extraction process is optimized,the main findings are as follows:1.UV detection of chlorogenic acid recovery was 101.12%,cv=0.51%,a correlation coefficient of 0.9979;liquid chromatography of RSD=1.45%,a correlation coefficient of 0.9998 shows that the two methods can be used to Ramie detection of chlorogenic acid, but as a result of expensive liquid chromatography,loss is too large,the current precision is used only for laboratory testing phase,UV detection adapted to batch.2.On the stability of chlorogenic acid have been studied,results showed that at room temperature,24h with more stable.Easier to decompose in alkaline conditions,pH=4 in the more stable under acidic conditions,chlorogenic acid in strong light conditions,easy to break down.Chlorogenic acid in the extraction and separation should be as much as possible to maintain the acidic,dark conditions.3.The best way to dry ramie samples:the first placed under the shade of ventilation 24h,then placed in 60℃constant temperature drying oven drying to constant weight 48h;chlorogenic acid as the optimum extraction solvent ethanol 70%;through the single-factor Experimental analysis of chlorogenic acid extracted from the best process parameters were:extraction time was 2 hours,the temperature is 70℃,pH value of 4.0, liquid ratio of 1:10.Analysis by orthogonal experiments,the factors on Ramie leaf extract of chlorogenic acid in the rate of descending order as follows:extraction temperature, extraction time,extraction pH,ratio of material to liquid.4.Ramie 25 candidates for a variety agronomic traits in field surveys,and examine the leaves of chlorogenic acid content,the content of a range of between 0.731%-0.059%, and so the initial six chlorogenic acid content is relatively high species,the highest concentration is on the 1st Gordon Limonene is Anren Debregeasia Huang chlorogenic acid content of 12 times.Chlorogenic acid content of ramie plant height and its correlation (R~2=0.0155),stem diameter correlation(R~2=0.0023) not significant.5.Through the experimental area and was informed that the ramie of different areas, different seasons Ramie-age of chlorogenic acid content is also different:in various parts of the root of the average level of the highest,0.538%,leaves the second,for the 0.494%; three-ramie’s higher than the first and second ramie;one-year-old ramie chlorogenic acid content is higher than the content of perennial ramie.更多还原