【作者】 蒲红伟；

【导师】 陈晓；

【作者基本信息】 新疆医科大学 ， 病理学与病理生理学， 2008， 硕士

【摘要】 目的研究肿瘤相关基因MCM-2、MTA-1、MDM-2和Grp94在食管鳞状细胞癌中的表达及意义。方法应用免疫组化S-P法检测食管鳞状细胞癌患者80例,,观察肿瘤相关基因MCM-2、MTA-1、MDM-2和Grp94的表达,同时回顾性研究了表达与食管鳞状细胞癌发生、浸润及转移等临床病理学参数的关系。结果(1)80例食管鳞状细胞癌组织中,MCM-2、MTA-1 MDM-2、和Grp94的表达阳性检出率分别为:50.0%(40/80)、70.0%(56/80)、67.5%(54/80)、68.8%(55/80)。(2)食管鳞状细胞癌组织中,不同分化程度MCM-2蛋白的阳性表达检出率分别为28.6%和61.5%。P<0.05,统计学差异有显著性。不同浸润深度的MCM-2蛋白的阳性表达检出率分别为41.2%和52.4%。Р>0.05,统计学差异无显著性。有、无淋巴结转移时,MCM-2蛋白的阳性表达检出率分别为81.0%和39.0%。两者比较, P<0.05,统计学差异有显著性。(3)食管鳞状细胞癌组织中,不同分化程度MTA-1蛋白的阳性表达检出率分别为71.4%和69.2%。两者比较,Р>0.05,统计学差异无显著性。食管鳞状细胞癌组织中,不同浸润深度的MTA-1蛋白的阳性表达检出率分别为64.7%和73.2%。两者比较, P<0.05,统计学差异有显著性。食管鳞状细胞癌组织中,有、无淋巴结转移时,MTA-1蛋白的阳性表达检出率分别为81.0%和66.1%。两者比较,Р>0.05,统计学差异无显著性。(4)食管鳞状细胞癌组织中,不同分化程度MDM-2蛋白的阳性表达检出率分别为42.9%和80.8%。两者比较, P<0.05,统计学差异有显著性。不同浸润深度的MDM-2蛋白的阳性表达检出率分别为52.9%和71.4%。两者比较,Р>0.05,统计学差异无显著性。有、无淋巴结转移时,MDM-2蛋白的阳性表达检出率分别为81.0%和62.7%。两者比较,Р>0.05,统计学差异无显著性。(5)食管鳞状细胞癌组织中,不同分化程度Grp-94蛋白的阳性表达检出率分别为53.6%和78.8%。两者比较, P<0.05,统计学差异有显著性。不同浸润深度的Grp-94蛋白的表达阳性表达检出率分别为52.9%和77.8%。两者比较,Р<0.05,统计学差异有显著性。有、无淋巴结转移时,Grp-94蛋白的阳性表达检出率分别为71.4%和69.5%。两者比较,Р>0.05,统计学差异无显著性。结论(1)提示,MCM-2、MTA-1、MDM-2和Grp94在肿瘤发生中起重要作用。(2)提示MCM-2蛋白对肿瘤的恶性程度判定、预后判定是一个敏感指标;MCM-2是与肿瘤转移关系密切的指标和标志物。(3)食管鳞状细胞癌组织中, MTA-1蛋白与肿瘤生长发展关系密切。(4)MDM-2蛋白是判定预后的重要标志物。(5)Grp-94蛋白的阳性表达是病理学分级和判定预后的重要标志物,与浸润生长关系密切。更多还原

【Abstract】 Objective To investigate clinical pathology significance and expression of MCM-2、MTA-1、MDM-2 and Grp94 in esophageal squamous cell carcinomant. Methods Envision immunohistochemical method was used to detect the expressions of MCM-2、MTA-1、MDM-2 and Grp94 in 80 cases of esophageal squamous cell carcinoma and in 15 cases of normal tissues, and to analyse the relationship between them and different clinical pathological factors. Results (1) The positive frequency of MCM-2、MTA-1、MDM-2 and Grp94 in esophageal squamous cell carcinoma tissues was separately 50.0%(40/80), 70.0%(56/80), 67.5%(54/80)and 68.8%(55/80).(2) The expressions of MCM-2 of differenciation in esophageal squamous cell carcinomant was 28.6% and 61.5%. Compared with them, there is difference between them (P<0.05). The expressions of MCM-2 of infiltratiion in esophageal squamous cell carcinomant was 41.2% and 52.4%. Compared with them,there is no difference between them (Р>0.05). The expressions of MCM-2of lymphnode metastasis in esophageal squamous cell carcinomant was 81.0% and 39.3%. Compared with them, there is difference between them (P<0.05).(3) The expressions of MTA-1 of differenciation in esophageal squamous cell carcinomant was 71.4% and 69.2%. Compared with them, there is no difference between them (P>0.05). The expressions of MTA-1 of infiltratiion in esophageal squamous cell carcinomant was 64.7% and 73.2%. Compared with them,there is difference between them (Р<0.05). The expressions of MTA-1 of lymphnode metastasis in esophageal squamous cell carcinomant was 81.0% and 66.1%. Compared with them, there is no difference between them (Р>0.05). (4) The expressions of MDM-2 of differenciation in esophageal squamous cell carcinomant was 42.9% and 80.8%. Compared with them, there is difference between them (P<0.05). The expressions of MDM-2 of infiltratiion in esophageal squamous cell carcinomant was 52.9% and 71.4%. Compared with them,there is no difference between them (Р>0.05). The expressions of MDM-2 of lymphnode metastasis in esophageal squamous cell carcinomant was 81.0% and 62.7%. Compared with them, there is no difference between them (Р>0.05). (5)The expressions of Grp-94 of differenciation in esophageal squamous cell carcinomant was 53.6% and 78.8%. Compared with them, there is difference between them (P<0.05). The expressions of Grp94 of infiltratiion in esophageal squamous cell carcinomant was 52.9% and 77.8%. Compared with them,there is difference between them (P<0.05). The expressions of Grp94 of lymphnode metastasis in esophageal squamous cell carcinomant was 71.4% and 69.5%. Compared with them, there is no difference between them (P>0.05). Conclusion (1) It is suggested that MCM-2、MTA-1、MDM-2 and Grp94 play a important role in pathogenesis. (2) It is suggested that MCM-2 was a marker of tumor maligness and prognosis. (3) The expression of MTA-1 had a closed relationship with development.(4)The expression of MDM-2 was a marker of prognosis.(5) Grp-94 was a marker of classification and prognosis, and had a closed relationship with infiltration.更多还原