【作者】 李维维；

【导师】 刘晓秋；

【作者基本信息】 沈阳药科大学 ， 生药学， 2006， 硕士

【摘要】 蒲黄为香蒲科植物水烛香蒲Typha angustifolia L．、东方香蒲Typha orientalis Presl或同属植物的干燥花粉，具有止血、化瘀、通淋功能。现代药理证明蒲黄具有改善微循环、抗心肌缺血和抑制动脉粥样硬化的作用，其主要活性成分为黄酮类成分。本文主要对蒲黄的化学对照品、质量控制方法，提取物的制备工艺进行研究，为中药蒲黄质量标准的修订和开发利用提供依据。1．化学对照品的研究：采用溶剂提取法、大孔树脂柱色谱、聚酰胺柱色谱、Sephadex LH-20柱色谱等手段，对蒲黄中的主要黄酮类成分进行分离鉴定，得到4个黄酮类化合物，分别为香蒲新苷(typhaneoside，1)、异鼠李素-3-O-新橙皮苷(isorhamnetin-3-O-neohesperidoside，2)、柚皮素(naringenin，3)和异鼠李素(isorhamnetin，4)。经TLC、HPLC分析，纯度分别为99.2％，99.7％，99.0％和97.2％，符合定性、定量用对照品的要求。2．质量综合评价：对21批不同来源蒲黄样品进行了系统的鉴别、检查和含量测定，并对其质量进行综合评价，为科学、合理地控制蒲黄药材的质量及新版药典中蒲黄质量标准的修订提供依据。主要内容如下：(1)通过性状和显微特征对其进行质量分级；建立了以香蒲新苷、异鼠李素-3-O-新橙皮苷和柚皮素为对照品的薄层色谱鉴别方法；建立了HPLC指纹图谱，指认其中4个成分，分别为香蒲新苷、异鼠李素-3-O-新橙皮苷、柚皮素和异鼠李素；(2)对蒲黄药材进行水分、总灰分检查和醇溶性浸出物测定；(3) HPLC法同时测定蒲黄中香蒲新苷、异鼠李素-3-O-新橙皮苷、柚皮素和异鼠李素的含量。结果香蒲新苷、异鼠李素-3-O-新橙皮苷、柚皮素和异鼠李素的线性范围分别为7.0～280μg／mL(r=0.9996)，16.0～288μg／mL(r=0.9998)，5.3～212μg／mL(r=0.9998)和2.0～125μg／mL(r=0.9994)；回收率分别为：100.3％(RSD=1.26％)，99.8％(RSD=1.80％)、99.9％(RSD=1.09％)和100.0％(RSD=1.26％)。3．提取物制备工艺研究：以香蒲新苷和异鼠李素-3-O-新橙皮苷的含量为指标，优化蒲黄提取物的提取和纯化工艺。更多还原

【Abstract】 Pollen Typhae is the dried pollen of Typha angustifolia L., Typha orientalis Presl or other plants of the same genus(Fam. Typhacae), which has action to arrest bleeding, to remove blood stasis, and to relieve dysuria. Pollen Typhae, in which flavoniods are the main active constituents, has action to improve the microcirculation, resist myocardial ischemia and inhibit artherosclerosis. To provide the basis for the quality assessment of pollen Typhae and the preparation technology of its extract, the flowing studies were carried out.1. Research on chemical constituents. Four compounds were separated from polle n Typhae with solvent extraction and column chromatograph such as Macroporous adso rption resin, polyamide and Sephadex LH-20 chromatography, and were identified as t yphaeneoside(l), isorhamnetin-3-O-neohesperidoside(2),naringenin(3) and isorhamnetin(4).Analyzed by HPLC, their purities were 99.2%, 99.7%, 99.0% and 97.2% respectivel y, which met the requirement of the standard substances.2. Quality assessment. Systematic researches and assessment on pollen Typhae collected from 21 different areas, including identification, quality examination and assaying, were performed. It provides basis for scientific and reasonable quality control of pollen Typhae and the revise of new pharmacopeia.(1) Quality classification according to their character of description and micrology was carried out. The method of TLC identication of pollen Typhae using typhaneoside, isorhamnetin-3-O-neohesperidoside, naringenin and isorhamnetin as reference substances. HPLC fingerprints was established, in which four peaks were identified respectively as typhaneoside, isorhamnetin-3-O-neohesperidoside, naringenin and isorhamnetin.( 2 ) Content of water, total ashes and ethanol-soluble extract were determined.(3) RP-HPLC method was developed for the determination of typhaeneoside, isorhamnetin-3-O-neohesperidoside, naringenin and isorhamnetin in pollen Typhae. The linear ranges for typhaeneoside, isorhamnetin-3-O-neohesperidoside, naringenin and isorhamnetin were 7.0～280μg/mL (r=0.9996), 16.0～288μg/mL (r=0.9998), 5.3～212μg/mL (r=0.9998) and 2.0～125μg/mL (r=0.9994), respectively. Their average recoveries were 100.3% (RSD=1.26%), 99.8% (RSD=1.80%)、99.9% (RSD=1.09 %) and 100.0% (RSD=1.26%), respectively.3. Research on the preparation method of pollen Typhae’s extract. Considering the content of typhaeneoside and isorhamnetin-3-O-neohesperidoside, the extraction and purification technology of pollen Typhae were optimized.更多还原