【作者】 王征；

【导师】 张晓明；

【作者基本信息】 浙江大学 ， 人体解剖与组织胚胎学， 2011， 硕士

【摘要】 糖尿病肾病(diabetic nephropathy, DN)是糖尿病的慢性微血管并发症之一,它引发的终末期肾功能衰竭(ESRD)是导致糖尿病患者死亡的主要原因,目前其具体发病机制尚未阐明。本文研究血小板反应蛋白(thrombospondin 1, TSP-1)、纤维连接蛋白(f ibronectin, FN)在链脲佐菌素诱导的糖尿病模型大鼠肾组织中的表达改变。选取成年SD雄性大鼠随机分为两组,每组12只,实验组用链脲佐菌素诱导产生糖尿病模型,正常对照组注射等量柠檬酸钠缓冲液。成模6周后,HE染色观察肾组织病理变化,免疫组织化学和实时荧光定量PCR观察各组大鼠肾TSP-1和FN的蛋白和mRNA的表达情况。免疫组织化学结果显示糖尿病大鼠肾组织中表达TSP-1和FN蛋白数量较正常对照组上升,差异有统计学意义。同时,实时荧光定量PCR观察到糖尿病大鼠肾组织中TSP-1的mRNA较正常对照组上升,差异有统计学意义,而糖尿病大鼠肾组织中FN的mRNA较正常对照组也有上升,但无统计学差异。上述研究结果表明,高血糖状态下,糖尿病大鼠肾组织TSP-1和FN的表达增加,可能是糖尿病肾病发生、发展的一个重要因素。更多还原

【Abstract】 Diabetic nephropathy is diabetic chronic complications of micrangium, however the pathological mechanism involved was still unknown. The thesis investigates the expression of thrombospondin-1 (TSP-1) and fibronectin (FN) in renal tissue of diabetic model rats. Adult Sprague-Dawley male rats were randomly divided into two groups. The group of diabetes were injected with streptozotocin and the others were injected with sodium citrate buffer as the group of control. After 6 weeks later, the change of renal histomorphology was observed by hematoxylin-eosin staining, the expression of TSP-1 and FN were quantified in renal tissue by immunohistochemistry and real time-reverse transcription-quantitative PCR.6 weeks later, when compared with the control rats, a signficiant incresae of TSP-1 and FN were observed by immunohistochemistry in the diabetic rats, the mRNA of TSP-1 was also strongly increase in the diabetic rats, the mRNA of FN was slightly higher compared with the control rats, but no significant difference. Our study provides experimental evidence that the TSP-1 and FN’ expression was up-regulatd in renal tissue of streptozotocin-induced diabetic rats under hyperglycemia condition. TSP-1 and FN may play a role in the pathophysiology of diabetic nephropathy.更多还原